Document Detail

Sizzled: a secreted Xwnt8 antagonist expressed in the ventral marginal zone of Xenopus embryos.
MedLine Citation:
PMID:  9428410     Owner:  NLM     Status:  MEDLINE    
An expression cloning screen was used to isolate a novel gene homologous to the extracellular cysteine-rich domain of frizzled receptors. The gene (which we called sizzled for secreted frizzled) was shown to encode a soluble secreted protein, containing a functional signal sequence but no transmembrane domains. Sizzled (szl) is capable of inhibiting Xwnt8 as assayed by (1) dose-dependent inhibition of siamois induction by Xwnt8 in animal caps, (2) rescue of embryos ventralized by Xwnt8 DNA and (3) inhibition of XmyoD expression in the marginal zone. Szl can dorsalize Xenopus embryos if expressed after the midblastula transition, strengthening the idea that zygotic expression of wnts and in particular of Xwnt8 plays a role in antagonizing dorsal signals. It also suggests that inhibiting ventralizing wnts parallels the opposition of BMPs by noggin and chordin. szl expression is restricted to a narrow domain in the ventral marginal zone of gastrulating embryos. szl thus encodes a secreted antagonist of wnt signaling likely involved in inhibiting Xwnt8 and XmyoD ventrally and whose restricted expression represents a new element in the molecular pattern of the ventral marginal zone.
A N Salic; K L Kroll; L M Evans; M W Kirschner
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  124     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  1997 Dec 
Date Detail:
Created Date:  1998-02-02     Completed Date:  1998-02-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  4739-48     Citation Subset:  IM    
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
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MeSH Terms
Amino Acid Sequence
Binding Sites
Drosophila Proteins*
Embryo, Nonmammalian / drug effects,  radiation effects
Frizzled Receptors
Gene Expression Regulation, Developmental
Membrane Proteins / genetics
Molecular Sequence Data
Proteins / genetics*,  metabolism*,  secretion*
Proto-Oncogene Proteins / metabolism*
Receptors, G-Protein-Coupled
Sequence Homology, Amino Acid
Ultraviolet Rays
Wnt Proteins
Xenopus Proteins*
Xenopus laevis / embryology*
Zebrafish Proteins*
Reg. No./Substance:
0/Drosophila Proteins; 0/FRZB protein; 0/Frizzled Receptors; 0/Glycoproteins; 0/Membrane Proteins; 0/Proteins; 0/Proto-Oncogene Proteins; 0/Receptors, G-Protein-Coupled; 0/Wnt Proteins; 0/Xenopus Proteins; 0/Zebrafish Proteins; 0/fz protein, Drosophila; 0/sizzled protein, Xenopus

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