| Sizing of the Haemophilus influenzae Rd genome by pulsed-field agarose gel electrophoresis. | |
| | |
MedLine Citation:
|
PMID: 2842319 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The four restriction enzymes ApaI (5'-GGGCCC), EagI (5'-CGGCCG), NaeI (5'-GCCGGC), and SmaI (5'-CCCGGG) were found to produce distributions of DNA fragment sizes useful for mapping of the Haemophilus influenzae Rd genome by pulsed-field agarose gel electrophoresis. ApaI produced 21 fragments (range, 1.6 to 305 kilobases [kb]), EagI yielded 30 fragments (0.6 to 339 kb), NaeI produced 32 fragments (2.3 to 290 kb), and SmaI yielded 16 fragments (6.0 to 377 kb). Summation of the fragment lengths in each digest yielded estimates for the size of the H. influenzae chromosome ranging from 1,834 kb. |
| | |
Authors:
|
J J Lee; H O Smith |
Related Documents
:
|
11090619 - Analysis of the okazaki fragment distributions along single long dnas replicated by the... 1881899 - Integration of dna fragments by illegitimate recombination in saccharomyces cerevisiae. 2862219 - Characterization of dna fragments encoding fimbriae of the uropathogenic escherichia co... 16789219 - Molecular cloning and physical mapping of restriction endonuclease fragments of autogra... 6277739 - Characterization of coliphage lambda hybrids carrying dna fragments from herpes simplex... 501339 - Physicochemical properties and restriction maps of simian adenovirus type 38 dna. 2744489 - Expression vector pt7:tkii for the synthesis of authentic biologically active rna encod... 8185309 - Dna strand breaks following in vitro exposure to asbestos increase with surface-complex... 23640759 - Supercoiled pseudocircular domains in single twisted dnas under tension. elastic consta... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Journal of bacteriology Volume: 170 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1988 Sep |
Date Detail:
|
Created Date: 1988-10-06 Completed Date: 1988-10-06 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 4402-5 Citation Subset: IM |
Affiliation:
|
Department of Molecular Biology and Genetics, Johns Hopkins University Medical School, Baltimore, Maryland 21205. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Chromosome Mapping Chromosomes, Bacterial DNA Restriction Enzymes DNA, Bacterial / genetics* Electrophoresis, Agar Gel Genes, Bacterial Haemophilus influenzae / genetics* |
| Chemical | |
Reg. No./Substance:
|
0/DNA, Bacterial; EC 3.1.21.-/DNA Restriction Enzymes |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Cloning of the Rhodobacter capsulatus hemA gene.
Next Document: Bacterial-type ferredoxin genes in the nitrogen fixation regions of the cyanobacterium Anabaena sp. ...