Document Detail


Sizing of the Haemophilus influenzae Rd genome by pulsed-field agarose gel electrophoresis.
MedLine Citation:
PMID:  2842319     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The four restriction enzymes ApaI (5'-GGGCCC), EagI (5'-CGGCCG), NaeI (5'-GCCGGC), and SmaI (5'-CCCGGG) were found to produce distributions of DNA fragment sizes useful for mapping of the Haemophilus influenzae Rd genome by pulsed-field agarose gel electrophoresis. ApaI produced 21 fragments (range, 1.6 to 305 kilobases [kb]), EagI yielded 30 fragments (0.6 to 339 kb), NaeI produced 32 fragments (2.3 to 290 kb), and SmaI yielded 16 fragments (6.0 to 377 kb). Summation of the fragment lengths in each digest yielded estimates for the size of the H. influenzae chromosome ranging from 1,834 kb.
Authors:
J J Lee; H O Smith
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  170     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1988 Sep 
Date Detail:
Created Date:  1988-10-06     Completed Date:  1988-10-06     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4402-5     Citation Subset:  IM    
Affiliation:
Department of Molecular Biology and Genetics, Johns Hopkins University Medical School, Baltimore, Maryland 21205.
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MeSH Terms
Descriptor/Qualifier:
Chromosome Mapping
Chromosomes, Bacterial
DNA Restriction Enzymes
DNA, Bacterial / genetics*
Electrophoresis, Agar Gel
Genes, Bacterial
Haemophilus influenzae / genetics*
Chemical
Reg. No./Substance:
0/DNA, Bacterial; EC 3.1.21.-/DNA Restriction Enzymes
Comments/Corrections

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