Document Detail

Size, stability, and entrapment efficiency of phospholipid nanocapsules containing polypeptide antimicrobials.
MedLine Citation:
PMID:  14690399     Owner:  NLM     Status:  MEDLINE    
The effect of lipid composition [phosphatidylcholine (PC), phosphatidylglycerol (PG), and cholesterol] on size, stability, and entrapment efficiency of polypeptide antimicrobials in liposomal nanocapsules was investigated. PC, PC/cholesterol (70:30), and PC/PG/cholesterol (50:20:30) liposomes had entrapment efficiencies with calcein of 71, 57, and 54% with particle sizes of 85, 133, and 145 nm, respectively. Co-encapsulation of calcein and nisin resulted in entrapment efficiencies of 63, 54, and 59% with particle sizes of 144, 223, and 167 nm for PC, PC/cholesterol (70:30), and PC/PG/cholesterol (50:20:30), respectively. Co-encapsulation of calcein and lysozyme yielded entrapment efficiencies of 61, 60, and 61% with particle sizes of 161, 162, and 174 nm, respectively. The highest concentration of antimicrobials was encapsulated in 100% PC liposomes. Nisin induced more calcein release compared to lysozyme. Results demonstrate that production and optimization of stable nanoparticulate aqueous dispersions of polypeptide antimicrobials for microbiological stabilization of food products depend on selection of suitable lipid-antimicrobial combinations.
Lilian M Were; Barry D Bruce; P Michael Davidson; Jochen Weiss
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  51     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-12-23     Completed Date:  2004-03-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8073-9     Citation Subset:  IM    
Department of Food Science and Technology, Walter Life Sciences, and Food Safety Center of Excellence, The University of Tennessee, Knoxville, TN 37996, USA.
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MeSH Terms
Anti-Bacterial Agents / administration & dosage*,  analysis*
Cholesterol / analysis
Drug Stability
Fluorescent Dyes
Food Microbiology*
Liposomes / chemistry*
Muramidase / administration & dosage
Nisin / administration & dosage
Particle Size
Phosphatidylcholines / analysis
Phosphatidylglycerols / analysis
Phospholipids / analysis*
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Fluoresceins; 0/Fluorescent Dyes; 0/Liposomes; 0/Phosphatidylcholines; 0/Phosphatidylglycerols; 0/Phospholipids; 1414-45-5/Nisin; 1461-15-0/fluorexon; 57-88-5/Cholesterol; EC

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