| Size separations of starch of different botanical origin studied by asymmetrical-flow field-flow fractionation and multiangle light scattering. | |
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MedLine Citation:
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PMID: 21181137 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Asymmetrical-flow field-flow fractionation combined with multiangle light scattering and refractive index detection has been revealed to be a powerful tool for starch characterization. It is based on size separation according to the hydrodynamic diameter of the starch components. Starch from a wide range of different botanical sources were studied, including normal starch and high-amylose and high-amylopectin starch. The starch was dissolved by heat treatment at elevated pressure in a laboratory autoclave. This gave clear solutions with no granular residues. Amylose retrogradation was prevented by using freshly dissolved samples. Programmed cross flow starting at 1.0 mL min(-1) and decreasing exponentially with a half-life of 4 min was utilised. The starches showed two size populations representing mainly amylose and mainly amylopectin with an overlapping region where amylose and amylopectin were possibly co-eluted. Most of the first population had molar masses below 10(6) g mol(-1), and most of the second size population had molar masses above 10(7) g mol(-1). Large differences were found in the relative amounts of the two populations, the molar mass, and hydrodynamic diameters, depending on the plant source and its varieties. |
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Authors:
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Karl-Gustav Wahlund; Mats Leeman; Stalin Santacruz |
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Publication Detail:
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Type: Journal Article Date: 2010-12-22 |
Journal Detail:
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Title: Analytical and bioanalytical chemistry Volume: 399 ISSN: 1618-2650 ISO Abbreviation: Anal Bioanal Chem Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101134327 Medline TA: Anal Bioanal Chem Country: Germany |
Other Details:
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Languages: eng Pagination: 1455-65 Citation Subset: IM |
Affiliation:
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Technical Analytical Chemistry, Department of Chemistry, Center for Chemistry and Chemical Engineering, Faculty of Engineering LTH, Lund University, P.O. Box 124, 221 00, Lund, Sweden, karl-gustav.wahlund@analykem.lu.se. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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