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Size exclusion and anion exchange high performance liquid chromatography for characterizing metals bound to marine dissolved organic matter.
MedLine Citation:
PMID:  23265737     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Size exclusion chromatography (SEC) followed by anion exchange chromatography (AEC) hyphenated with inductively coupled plasma-mass spectrometry (ICP-MS) was applied for fractionating metals bound to marine dissolved organic matter (DOM). Surface seawater samples (100L) were subjected to tangential flow ultrafiltration (10,000Da cut off) for isolating and pre-concentrating dissolved large molecules. The isolated fraction (retentate) consisted of 1L, which was further freeze-dried and re-dissolved to 250mL with ultrapure water. After HI Trap desalting of the re-dissolved retentate, SEC with UV detection showed marine DOM ranging from 6.5kDa (lower than the permeable volume of the SEC column) to 16kDa. A further characterization of this fraction by AEC with UV detection revealed the existence of four groups of macromolecules exhibiting retention times of 2.3, 2.8, 4.5 and 14.0min. AEC hyphenated with ICP-MS showed the presence of strontium and zinc in the first AE fraction isolated from the SEC fraction; while manganese was found to be bound to the second AE fraction. Cobalt was found to be bound to molecules comprising the third AE fraction.
Authors:
Natalia García-Otero; Pilar Bermejo-Barrera; Antonio Moreda-Piñeiro
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Publication Detail:
Type:  Journal Article     Date:  2012-11-23
Journal Detail:
Title:  Analytica chimica acta     Volume:  760     ISSN:  1873-4324     ISO Abbreviation:  Anal. Chim. Acta     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370534     Medline TA:  Anal Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  83-92     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Affiliation:
Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemistry, University of Santiago de Compostela, Avenida das Ciencias, s/n, 15782 Santiago de Compostela, Spain.
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