| Size exclusion and anion exchange high performance liquid chromatography for characterizing metals bound to marine dissolved organic matter. | |
| | |
MedLine Citation:
|
PMID: 23265737 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Size exclusion chromatography (SEC) followed by anion exchange chromatography (AEC) hyphenated with inductively coupled plasma-mass spectrometry (ICP-MS) was applied for fractionating metals bound to marine dissolved organic matter (DOM). Surface seawater samples (100L) were subjected to tangential flow ultrafiltration (10,000Da cut off) for isolating and pre-concentrating dissolved large molecules. The isolated fraction (retentate) consisted of 1L, which was further freeze-dried and re-dissolved to 250mL with ultrapure water. After HI Trap desalting of the re-dissolved retentate, SEC with UV detection showed marine DOM ranging from 6.5kDa (lower than the permeable volume of the SEC column) to 16kDa. A further characterization of this fraction by AEC with UV detection revealed the existence of four groups of macromolecules exhibiting retention times of 2.3, 2.8, 4.5 and 14.0min. AEC hyphenated with ICP-MS showed the presence of strontium and zinc in the first AE fraction isolated from the SEC fraction; while manganese was found to be bound to the second AE fraction. Cobalt was found to be bound to molecules comprising the third AE fraction. |
| | |
Authors:
|
Natalia García-Otero; Pilar Bermejo-Barrera; Antonio Moreda-Piñeiro |
Related Documents
:
|
23327897 - On the formation of bromhexine impurity e and its chromatographic behaviour. 22946537 - Chemical constituents of the leaf of alpinia mutica roxb. 8181427 - Influence of pathologic scotopization on the extended rayleigh match. 23200367 - Arsenic speciation in water and snow samples by adsorption onto phema in a micro-pipett... 21111427 - Validation of a liquid chromatography method for the simultaneous quantification of och... 3876897 - Determination of alpha-1-antitrypsin phenotypes and m subtypes by isoelectric focusing ... |
Publication Detail:
|
Type: Journal Article Date: 2012-11-23 |
Journal Detail:
|
Title: Analytica chimica acta Volume: 760 ISSN: 1873-4324 ISO Abbreviation: Anal. Chim. Acta Publication Date: 2013 Jan |
Date Detail:
|
Created Date: 2012-12-25 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0370534 Medline TA: Anal Chim Acta Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 83-92 Citation Subset: IM |
Copyright Information:
|
Copyright © 2012 Elsevier B.V. All rights reserved. |
Affiliation:
|
Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemistry, University of Santiago de Compostela, Avenida das Ciencias, s/n, 15782 Santiago de Compostela, Spain. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Simple approach to study biomolecule adsorption in polymeric microfluidic channels.
Next Document: Simultaneous determination of 3-monochloropropane-1,2-diol and acrylamide in food by gas chromatogra...