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Size distribution of luteal cells during pseudopregnancy in domestic cats.
MedLine Citation:
PMID:  19000225     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Experiments were designed to investigate the size distribution of queen steroidogenic luteal cells throughout pseudopregnancy. Corpora lutea were obtained from the queens following ovariohysterectomy on days 7, 15 or 25 of pseudopregnancy. Luteal cells were isolated from the ovary by collagenase digestion. Steriodogenic cells were identified by staining of cells for 3beta-HSD activity. Cell diameters were measured using a microscope. Luteal cells having steroidogenic capacity covered a wide spectrum of sizes ranging from 3 to 35 mum in diameter. There was a significant increase in mean cell diameters (p < 0.01) as pseudopregnancy progressed. Mean diameter of 3beta-HSD positive cells increased from 10.41 +/- 0.7 microm, on day 7 of pseudopregnancy, to 19.72 +/- 1.3 microm on day 25 of pseudopregnancy. The ratio of large (>20 microm in diameter) to small (3-20 microm in diameter) luteal cells was 0.08 : 1.0 on day 7 of pseudopregnancy, with the 7.5-10 microm cell size class predominant. By day 25 of pseudopregnancy, the ratio of large-to-small cells was increased to 0.87 : 1.0, and 20-25 microm cell sizes become predominant. In conclusion, this study has demonstrated that the cells of the corpus luteum undergo continuous differentiation during pseudopregnancy in queen. This study also demonstrates that luteal cells dissociated from pseudopregnant queen can be used as a model to study the physiology of corpus luteum in pregnant cats.
Authors:
S Arikan; A A Yigit; H Kalender
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-11-09
Journal Detail:
Title:  Reproduction in domestic animals = Zuchthygiene     Volume:  44     ISSN:  1439-0531     ISO Abbreviation:  Reprod. Domest. Anim.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-09-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9015668     Medline TA:  Reprod Domest Anim     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  842-5     Citation Subset:  IM    
Affiliation:
Department of Physiology, Faculty of Veterinary Medicine, University of Kirikkale, Kirikkale, Turkey. sevket95@hotmail.com
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