| Size distribution of liposomes by flow field-flow fractionation. | |
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MedLine Citation:
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PMID: 8305595 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The applicability of field-low fractionation (FFF) to the characterization of liposomes is discussed and theoretically described. Because of fundamental differences in their driving forces, sedimentation FFF and flow FFF measure different vesicle properties. Sedimentation FFF, although used previously to measure vesicle sizes and size distributions, is fundamentally a technique that measures the effective mass and mass distribution of particles. It is sensitive to small changes in the effective mass of either the biomembrane or its encapsulated load and thus is likely to be useful in characterizing such properties as drug loading, biomembrane volumes and areas, and distributions of these properties. Size characterization by sedimentation FFF can only be done by deducing size from effective mass. Flow FFF, by contrast, provides a direct measurement of vesicle size and size distribution. After demonstrating the high resolution and relative accuracy of size measurement of flow FFF by the separation of polystyrene latex standards, flow FFF was applied to two preparations of DSPC-DSPA liposomes that were sonicated under different temperature conditions. Fractograms and size distributions are reported as a function of sonication time. The rapid elimination of a large diameter tail to the distribution is shown to constitute a major mechanism for distribution narrowing. Finally, results are provided bearing on the reproducibility of size distribution measurements by flow FFF. |
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Authors:
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M H Moon; J C Giddings |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of pharmaceutical and biomedical analysis Volume: 11 ISSN: 0731-7085 ISO Abbreviation: J Pharm Biomed Anal Publication Date: 1993 Oct |
Date Detail:
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Created Date: 1994-03-11 Completed Date: 1994-03-11 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8309336 Medline TA: J Pharm Biomed Anal Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 911-20 Citation Subset: IM |
Affiliation:
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Department of Chemistry, University of Utah, Salt Lake City 84112. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chemical Fractionation
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methods* Liposomes / analysis* Models, Theoretical Particle Size |
| Grant Support | |
ID/Acronym/Agency:
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GM10851-35/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Liposomes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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