Document Detail

Size determination of bacterial capsular oligosaccharides used to prepare conjugate vaccines against Neisseria meningitidis groups Y and W135.
MedLine Citation:
PMID:  15734061     Owner:  NLM     Status:  MEDLINE    
The glycoconjugate vaccines against Neisseria meningitidis groups Y and W135 consist of pools of selected oligosaccharides conjugated to the protein carrier (CRM197). Consistent production of these vaccines requires control and thus determination of the average degree of polymerisation of the oligosaccharides used for conjugation. Acid hydrolysis generates group Y and W135 oligosaccharides with N-acetylneuraminic acid at the reducing end. A method, involving NaBH4 reduction and quantification of this terminal N-acetylneuraminic acid by use of high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) following acid hydrolysis (2M TFA), was developed. The average degree of polymerisation is calculated from the ratio of reduced N-acetylneuraminic acid to total N-acetylneuraminic acid. The assay was qualified by application to group C, Y and W135 oligosaccharide standards characterised by liquid chromatography, mass and NMR spectroscopy.
Angela Bardotti; Giovanni Averani; Francesco Berti; Stefania Berti; Chiara Galli; Sara Giannini; Barbara Fabbri; Daniela Proietti; Neil Ravenscroft; Stefano Ricci
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Vaccine     Volume:  23     ISSN:  0264-410X     ISO Abbreviation:  Vaccine     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-02-28     Completed Date:  2005-05-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8406899     Medline TA:  Vaccine     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1887-99     Citation Subset:  IM    
Chiron Vaccines, Via Fiorentina 1, I-53100 Siena, Italy.
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MeSH Terms
Carbohydrate Sequence
Chemistry, Physical
Indicators and Reagents
Magnetic Resonance Spectroscopy
Meningococcal Vaccines / chemistry,  immunology*
Molecular Sequence Data
N-Acetylneuraminic Acid / chemistry
Neisseria meningitidis / classification,  immunology*
Oligosaccharides / chemistry*,  immunology*
Physicochemical Phenomena
Polysaccharides / chemistry,  immunology
Reference Standards
Spectrometry, Mass, Electrospray Ionization
Vaccines, Conjugate / chemistry,  immunology
Reg. No./Substance:
0/Indicators and Reagents; 0/Meningococcal Vaccines; 0/Oligosaccharides; 0/Polysaccharides; 0/Vaccines, Conjugate; 131-48-6/N-Acetylneuraminic Acid

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