| Size determination of bacterial capsular oligosaccharides used to prepare conjugate vaccines against Neisseria meningitidis groups Y and W135. | |
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MedLine Citation:
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PMID: 15734061 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The glycoconjugate vaccines against Neisseria meningitidis groups Y and W135 consist of pools of selected oligosaccharides conjugated to the protein carrier (CRM197). Consistent production of these vaccines requires control and thus determination of the average degree of polymerisation of the oligosaccharides used for conjugation. Acid hydrolysis generates group Y and W135 oligosaccharides with N-acetylneuraminic acid at the reducing end. A method, involving NaBH4 reduction and quantification of this terminal N-acetylneuraminic acid by use of high performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) following acid hydrolysis (2M TFA), was developed. The average degree of polymerisation is calculated from the ratio of reduced N-acetylneuraminic acid to total N-acetylneuraminic acid. The assay was qualified by application to group C, Y and W135 oligosaccharide standards characterised by liquid chromatography, mass and NMR spectroscopy. |
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Authors:
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Angela Bardotti; Giovanni Averani; Francesco Berti; Stefania Berti; Chiara Galli; Sara Giannini; Barbara Fabbri; Daniela Proietti; Neil Ravenscroft; Stefano Ricci |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Vaccine Volume: 23 ISSN: 0264-410X ISO Abbreviation: Vaccine Publication Date: 2005 Mar |
Date Detail:
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Created Date: 2005-02-28 Completed Date: 2005-05-02 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8406899 Medline TA: Vaccine Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1887-99 Citation Subset: IM |
Affiliation:
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Chiron Vaccines, Via Fiorentina 1, I-53100 Siena, Italy. angela_bardotti@chiron.it |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carbohydrate Sequence Chemistry, Physical Hydrolysis Indicators and Reagents Magnetic Resonance Spectroscopy Meningococcal Vaccines / chemistry, immunology* Molecular Sequence Data N-Acetylneuraminic Acid / chemistry Neisseria meningitidis / classification, immunology* Oligosaccharides / chemistry*, immunology* Oxidation-Reduction Physicochemical Phenomena Polysaccharides / chemistry, immunology Reference Standards Spectrometry, Mass, Electrospray Ionization Vaccines, Conjugate / chemistry, immunology |
| Chemical | |
Reg. No./Substance:
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0/Indicators and Reagents; 0/Meningococcal Vaccines; 0/Oligosaccharides; 0/Polysaccharides; 0/Vaccines, Conjugate; 131-48-6/N-Acetylneuraminic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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