| Size-dependent chromatographic separation of nucleic acids. | |
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MedLine Citation:
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PMID: 8227257 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Chromatographic procedures currently used for the size-dependent fractionation of nucleic acids are reviewed. First, an attempt is made to clarify the concept of "size" of nucleic acids and then various aspects of the chromatography of nucleic acids are considered. It is emphasized that consideration of the dynamic three-dimensional structure of large polynucleotides in a rapidly flowing eluent is essential for both the better understanding of mechanism and the development of sophisticated procedures. Of the practical chromatographic techniques that are not based on true size fractionation, ion-exchange chromatography on non-porous column packings appears to be the most efficient. Other methods, such as hydrophobic interaction, are unlikely to become popular. As for truly size-dependent modes, there are gel permeation and slalom chromatography. Although media with extremely large pores become available, the efficiency of gel permeation is still low as a practical separation procedure for large nucleic acid molecules. Its best use seems to be in the field of physicochemical research into nucleic acids in solution. The newly discovered slalom chromatography is based on a principle completely different from all other chromatographic modes. It enables the efficient separation of large double-stranded DNA fragments of 5-50 kilo base pairs by discriminating their length. It has proved not only to be useful as a tool for nucleic acid research but also to have great significance in other fields, e.g. the hydrodynamics of polymer solutions, the search for new chromatographic modes, etc. |
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Authors:
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K Kasai |
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Publication Detail:
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Type: Journal Article; Review |
Journal Detail:
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Title: Journal of chromatography Volume: 618 ISSN: 0021-9673 ISO Abbreviation: J. Chromatogr. Publication Date: 1993 Aug |
Date Detail:
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Created Date: 1993-12-07 Completed Date: 1993-12-07 Revised Date: 2005-11-16 |
Medline Journal Info:
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Nlm Unique ID: 0427043 Medline TA: J Chromatogr Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 203-21 Citation Subset: IM |
Affiliation:
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Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromatography, Gel / methods* Chromatography, Ion Exchange / methods* Humans Nucleic Acids / analysis*, chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Nucleic Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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