| Size-based separation and collection of mouse pancreatic islets for functional analysis. | |
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MedLine Citation:
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PMID: 20549367 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Islet size has recently been demonstrated to be an important factor in determining human islet transplantation outcomes. In this study, a multi-layered microfluidic device was developed and quantified for size-based separation of a heterogeneous population of mouse islets. The device was fabricated using standard soft lithography and polydimethylsiloxane (PDMS). Size-based separation was first demonstrated via injection of a heterogeneous population of glass beads between 50-300 microm in diameter which were separated into five sub-populations based on their diameter. Next, a heterogeneous population of mouse pancreatic islets, between 50-250 microm in diameter was separated into four sub-populations. Throughout this process the islets remained intact without any signs of damage, as indicated by cell viability staining. Islet glucose-stimulated insulin secretion of each sub-population of islets was also evaluated demonstrating that islets smaller than 150 microm have superior stimulation indexes (SI) compared to islets larger than 150 microm. In this study, we found that islets between 100 microm and 150 microm in diameter had the greatest SI value in a heterogeneous population of islets. |
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Authors:
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Ki-Hwan Nam; Wang Yong; Tricia Harvat; Adeola Adewola; Shesun Wang; Jose Oberholzer; David T Eddington |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biomedical microdevices Volume: 12 ISSN: 1572-8781 ISO Abbreviation: Biomed Microdevices Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-08-05 Completed Date: 2011-01-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100887374 Medline TA: Biomed Microdevices Country: United States |
Other Details:
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Languages: eng Pagination: 865-74 Citation Subset: IM |
Affiliation:
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Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60607, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Survival Glass / chemistry Glucose / pharmacology Gravitation Humans Insulin / secretion Islets of Langerhans* / cytology, drug effects, physiology, secretion Mice Microfluidic Analytical Techniques* Microspheres Organ Size Reproducibility of Results |
| Chemical | |
Reg. No./Substance:
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11061-68-0/Insulin; 50-99-7/Glucose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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