| Six novel genes necessary for pre-mRNA splicing in Saccharomyces cerevisiae. | |
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MedLine Citation:
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PMID: 8604335 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have identified six new genes whose products are necessary for the splicing of nuclear pre-mRNA in the yeast Saccharomyces cerevisiae. A collection of 426 temperature-sensitive yeast strains was generated by EMS mutagenesis. These mutants were screened for pre-mRNA splicing defects by an RNA gel blot assay, using the intron- containing CRY1 and ACT1 genes as hybridization probes. We identified 20 temperature-sensitive mutants defective in pre-mRNA splicing. Twelve appear to be allelic to the previously identified prp2, prp3, prp6, prp16/prp23, prp18, prp19 or prp26 mutations that cause defects in spliceosome assembly or the first or second step of splicing. One is allelic to SNR14 encoding U4 snRNA. Six new complementation groups, prp29-prp34, were identified. Each of these mutants accumulates unspliced pre-mRNA at 37 degrees C and thus is blocked in spliceosome assembly or early steps of pre-mRNA splicing before the first cleavage and ligation reaction. The prp29 mutation is suppressed by multicopy PRP2 and displays incomplete patterns of complementation with prp2 alleles, suggesting that the PRP29 gene product may interact with that of PRP2. There are now at least 42 different gene products, including the five spliceosomal snRNAs and 37 different proteins that are necessary for pre-mRNA splicing in Saccharomyces cerevisiae. However, the number of yeast genes identifiable by this approach has not yet been exhausted. |
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Authors:
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J R Maddock; J Roy; J L Woolford |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Nucleic acids research Volume: 24 ISSN: 0305-1048 ISO Abbreviation: Nucleic Acids Res. Publication Date: 1996 Mar |
Date Detail:
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Created Date: 1996-05-16 Completed Date: 1996-05-16 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0411011 Medline TA: Nucleic Acids Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1037-44 Citation Subset: IM |
Affiliation:
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Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213 USA. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Alleles Cell Nucleus / metabolism DEAD-box RNA Helicases DNA Probes Fungal Proteins / genetics, metabolism Genes, Fungal* Genes, Lethal Genetic Complementation Test Mutation Phenotype RNA Precursors / metabolism* RNA Splicing / genetics* RNA, Fungal / metabolism* Saccharomyces cerevisiae / genetics*, metabolism* Saccharomyces cerevisiae Proteins* Spliceosomes / genetics, metabolism Temperature |
| Grant Support | |
ID/Acronym/Agency:
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CA01000/CA/NCI NIH HHS; GM38782/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA Probes; 0/Fungal Proteins; 0/RNA Precursors; 0/RNA, Fungal; 0/Saccharomyces cerevisiae Proteins; EC 3.6.1.-/DEAD-box RNA Helicases; EC 3.6.1.-/PRP2 protein, S cerevisiae |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): Nucleic Acids Res ISSN: 0305-1048 ISSN: 1362-4962 |
Article Information Download PDF  Print publication date: Day: 15 Month: 3 Year: 1996 Volume: 24 Issue: 6 First Page: 1037 Last Page: 1044 ID: 145760 PubMed Id: 8604335 Publisher Item Identifier: 5t0770 |
| Six novel genes necessary for pre-mRNA splicing in Saccharomyces cerevisiae. | |
| J R Maddock | |
| J Roy | |
| J L Woolford, Jr | |
| Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213 USA. |
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