| Sitosterol reduces messenger RNA and protein expression levels of Niemann-Pick C1-like 1 in FHs 74 Int cells. | |
| | |
MedLine Citation:
|
PMID: 19963159 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
Intake of plant sterols has long been shown to reduce cholesterol absorption and subsequently plasma cholesterol concentrations. Despite competition between plant sterols and cholesterol for incorporation into mixed micelles as a suggested major mechanism for the inhibition of cholesterol absorption by plant sterols, studies exist to support an alternative mechanism. For example, another mechanism may be the action of plant sterols to reduce cholesterol absorption at the cellular level. This study was undertaken to test the hypothesis that plant sterols can modulate the expression of transporters such as Niemann-Pick C1-like 1 (NPC1L1) and scavenger receptor class B, type I (SR-BI) to lower intestinal cholesterol absorption. FHs 74 Int cells, a human small intestine epithelial cell line, were used as a model of enterocytes. The cells were treated with 25alpha-hydroxycholesterol (25 micromol/L) or 250 micromol/L of sitosterol, stigmasterol, and cholesterol for 24 hours to measure genes involved in cholesterol absorption and metabolism by quantitative real-time polymerase chain reaction. 25Alpha-hydroxycholesterol, cholesterol, and sitosterol significantly reduced the messenger RNA (mRNA) expression of NPC1L1 and hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, whereas SR-BI mRNA was not altered by the sterols. Western blot analysis confirmed the reduction in NPC1L1 by sterols. Depletion of cellular cholesterol by mevinolin, a cholesterol synthesis inhibitor, increased NPC1L1 and HMG-CoA reductase mRNA; and repletion of cholesterol abolished the increase. Sitosterol, but not stigmasterol, reduced the mRNA levels of NPC1L1 and HMG-CoA reductase to a similar extent of cholesterol. In conclusion, sitosterol can inhibit the expression of NPC1L1 in the enterocytes, which could be an alternate mechanism for plant sterols to reduce intestinal cholesterol uptake. |
| | |
Authors:
|
Elliot D Jesch; Jeong Min Seo; Timothy P Carr; Ji-Young Lee |
Related Documents
:
|
16216719 - Isocaloric substitution of plant sterol-enriched fat spread for carbohydrate-rich foods... 1478439 - Formation of ecdysteroids by y-organs of the crab, menippe mercenaria. i. biosynthesis ... 16772539 - Disposition and sterol-lowering effect of ezetimibe in multidrug resistance-associated ... 19772679 - Effect of rapeseed oil-derived plant sterol and stanol esters on atherosclerosis parame... 2836189 - Influence of exchanging polyunsaturated fat for carbohydrate on bile acid and cholester... 7988779 - Serum cholesterol and cholesterol and lipoprotein metabolism in hypercholesterolaemic n... 3775659 - Gallbladder contractility and mucus secretion after cholesterol feeding in the prairie ... 10828089 - Characterization and functional studies of lipoproteins, lipid transfer proteins, and l... 16061509 - Manipulation of ethanol production in anoxic rice coleoptiles by exogenous glucose dete... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
|
Title: Nutrition research (New York, N.Y.) Volume: 29 ISSN: 1879-0739 ISO Abbreviation: Nutr Res Publication Date: 2009 Dec |
Date Detail:
|
Created Date: 2009-12-07 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8303331 Medline TA: Nutr Res Country: United States |
Other Details:
|
Languages: eng Pagination: 859-66 Citation Subset: IM |
Affiliation:
|
Department of Nutrition and Health Sciences, University of Nebraska, Lincoln, NE 68583, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Pequi fruit (Caryocar brasiliense Camb.) pulp oil reduces exercise-induced inflammatory markers and ...
Next Document: Thiamine tetrahydrofurfuryl disulfide improves energy metabolism and physical performance during phy...