| Sites of allosteric shift in the structure of the cyclic AMP receptor protein. | |
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MedLine Citation:
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PMID: 2988785 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have characterized crp mutations in E. coli that allow CRP to function without cAMP. crp* mutants carrying a deletion of the gene encoding adenylate cyclase (cya) show significant lac expression. Cyclic GMP, normally an ineffective activator of CRP+, can stimulate these mutant CRP*s to permit greater lac expression in vivo. Cyclic AMP binding to the amino-terminal domain of CRP+ induces an allosteric transition that changes the DNA-binding property of the carboxy domain. The CRP* phenotype is caused by substitution of amino acids with bulkier side chains in the D alpha-helix of the protein's carboxy domain, near the hinge connecting the two domains. These results are consistent with a model in which the mutant CRP*s assume, in part, a conformation normally evoked only by cAMP binding: one in which the relative orientation of the C, D, and F alpha-helices is altered. We define precisely the amino acids of these alpha-helices that interact to cause the allosteric shift. |
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Authors:
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S Garges; S Adhya |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cell Volume: 41 ISSN: 0092-8674 ISO Abbreviation: Cell Publication Date: 1985 Jul |
Date Detail:
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Created Date: 1985-07-31 Completed Date: 1985-07-31 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0413066 Medline TA: Cell Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 745-51 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Base Sequence Cyclic AMP / metabolism, pharmacology* Cyclic GMP / pharmacology Escherichia coli / analysis Genes Mutation Phenotype Protein Conformation / drug effects Receptors, Cyclic AMP* / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Cyclic AMP; 60-92-4/Cyclic AMP; 7665-99-8/Cyclic GMP |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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