| Sites of persistence of lumpy skin disease virus in the genital tract of experimentally infected bulls. | |
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MedLine Citation:
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PMID: 19055553 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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The objectives of this work were to determine the site of persistence of lumpy skin disease virus (LSDV) in bulls shedding the virus in semen for a period longer than 28 days, to determine if the virus is present in all fractions of semen and to study lesions that developed in the genital tract. Six serologically negative postpubertal bulls were experimentally infected with a virulent field isolate of LSDV. The polymerase chain reaction (PCR) was performed on sheath washes, vesicular fluid, supernatant and cell-rich fractions of semen from day 10 to day 26 postinfection (p.i.). Bulls that were positive by PCR on the whole semen sample collected on day 28 p.i. were slaughtered and tissue samples from their genital tracts submitted for histopathological evaluation, immunoperoxidase staining, virus isolation and PCR. Two of the bulls developed severe lumpy skin disease (LSD) and were found to be shedding viral DNA in their semen on day 28 p.i. Viral DNA was identified in all semen fractions from all bulls, but mostly from the cell-rich fraction and from the severely affected bulls. The PCR assay was positive on postmortem samples of testes and epididymides from the two severely affected bulls. Virus could be recovered from the testes of these two bulls and from the epididymis of one of them. Immunoperoxidase staining was positive for LSDV staining in sections of testes and epididymides exhibiting necrosis. This study suggests that the testis and epididymis are sites of persistence of LSDV in bulls shedding virus in semen for prolonged periods and revealed that viral DNA is present in all fractions of the ejaculate. |
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Authors:
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C H Annandale; P C Irons; V P Bagla; U I Osuagwuh; E H Venter |
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Publication Detail:
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Type: Journal Article Date: 2008-12-02 |
Journal Detail:
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Title: Reproduction in domestic animals = Zuchthygiene Volume: 45 ISSN: 1439-0531 ISO Abbreviation: Reprod. Domest. Anim. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-07-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9015668 Medline TA: Reprod Domest Anim Country: Germany |
Other Details:
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Languages: eng Pagination: 250-5 Citation Subset: IM |
Affiliation:
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Section Reproduction, Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa. henry.annandale@up.ac.za |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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