| Site-specific thromboembolism: a novel animal model for stroke. | |
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MedLine Citation:
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PMID: 14970041 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND AND PURPOSE: To develop a technique for site-specific placement of a thrombus of predetermined volume in an animal model for the purpose of evaluating methods of intravascular thrombolysis and clot retrieval. METHODS: Six swine were subjected to thrombus injection bilaterally in the ascending pharyngeal artery (APA). Each animal underwent transfemoral angiography while under general anesthesia. A nondetachable balloon catheter and a 3-French microcatheter were then advanced into the common carotid artery through a 7-French guide catheter. With the microcatheter in the proximal APA and the balloon inflated proximally, a bolus of preformed thrombus composed of 0.9 mL of autologous blood and 0.1 mL of bovine thrombin (200 IU/mL) was injected through the microcatheter while local flow arrest was maintained for 15 min. The balloon was deflated and removed. The occluded arteries were observed by serial angiography for 3 hr and then resected for gross examination and hematoxylin and eosin staining. RESULTS: Each APA was occluded angiographically and did not recanalize during the 3-hr observation period. Persistent, proximal progression of thrombus to the superior thyroid artery origin occurred in three animals. Gross inspection revealed that the resected arteries contained thrombus in the proximal APA but not in the common carotid artery. Histologic examination revealed organized thrombus, without evidence of intimal injury. CONCLUSION: Our model provides a simple, reliable method for site-specific injection of a thrombus of predetermined volume. Site-specific placement is important for evaluation of the efficacy of thrombolytic agents and techniques. Angiographic evidence of brain revascularization can be used to grade revascularization and clot volume. The ability to specifically localize and estimate clot volume makes our model well suited for the evaluation and comparison of thrombolytic agents and endovascular techniques. |
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Authors:
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Andrew J Ringer; Lee R Guterman; L Nelson Hopkins |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: AJNR. American journal of neuroradiology Volume: 25 ISSN: 0195-6108 ISO Abbreviation: AJNR Am J Neuroradiol Publication Date: 2004 Feb |
Date Detail:
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Created Date: 2004-02-18 Completed Date: 2004-03-29 Revised Date: 2008-02-14 |
Medline Journal Info:
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Nlm Unique ID: 8003708 Medline TA: AJNR Am J Neuroradiol Country: United States |
Other Details:
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Languages: eng Pagination: 329-32 Citation Subset: IM |
Affiliation:
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Department of Neurosurgery and Toshiba Stroke Research Center, School of Medicine and Biomedical Sciences, University at Buffalo, State University at New York, Buffalo, NY, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Angiography, Digital Subtraction* Angioplasty, Balloon* Animals Arteries / pathology Balloon Dilatation Carotid Arteries / pathology Carotid Artery Thrombosis / drug therapy*, pathology, radiography Cerebral Angiography* Disease Models, Animal* Intracranial Embolism / drug therapy*, pathology, radiography Pharynx / blood supply Swine Thrombolytic Therapy* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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