|Site-specific phosphorylation of SCG10 in neuronal plasticity: role of Ser73 phosphorylation by N-methyl D-aspartic acid receptor activation in rat hippocampus.|
|PMID: 16368189 Owner: NLM Status: MEDLINE|
|Accumulated evidence suggests that actin and microtubule regulating proteins contribute to neuronal structural dynamics, which subsequently affect neuronal plasticity. SCG10 is a neuronal-specific stathmin protein with microtubule destabilizing activity that is affected by multiple phosphorylation, at least in vitro. SCG10 has four major phosphorylation sites: Ser50 and Ser97 targeted by protein kinase A (PKA), and Ser62 and Ser73 targeted by mitogen-activated protein kinase (MAPK). To explore the potential roles of site-specific phosphorylation in physiological models, we developed phosphorylation site-specific antibodies and examined the SCG10 status in primary cultured hippocampal neurons and tissues. Although SCG10 is concentrated in growth cones and the Golgi apparatus in primary cultured neurons, the phosphorylated form was also detected in both regions, suggesting that MT dynamics within the growth cone may be regulated by protein phosphorylation. In the adult hippocampus, an intense stimulus such as kainate treatment induced a rapid phosphorylation of Ser73 within 15 min that was sustained for at least 60 min. This response was mediated through the N-methyl D-aspartic acid (NMDA) receptor and was ablated by the antagonist MK-801. The MAPK enzyme Erk2 was simultaneously activated along a similar time course to SCG10, suggesting that Erk2 may directly phosphorylate Ser73. These results demonstrate that changes in the phosphorylation status of SCG10 in vivo, dependent upon neural activity and/or plasticity, could affect the microtubule dynamics in neuronal dendrites.|
|Hiroshi Morii; Tomiko Yamada; Itsuko Nakano; Judy M Coulson; Nozomu Mori|
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|Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2005-12-20|
|Title: Neuroscience letters Volume: 396 ISSN: 0304-3940 ISO Abbreviation: Neurosci. Lett. Publication Date: 2006 Apr|
|Created Date: 2006-03-06 Completed Date: 2006-05-25 Revised Date: 2009-11-19|
Medline Journal Info:
|Nlm Unique ID: 7600130 Medline TA: Neurosci Lett Country: Ireland|
|Languages: eng Pagination: 241-6 Citation Subset: IM|
|Department of Molecular Genetics, National Institute for Longevity Sciences, 36-3 Gengo, Morioka, Oobu, 474-8522, Japan.|
|APA/MLA Format Download EndNote Download BibTex|
Amino Acid Sequence
Dizocilpine Maleate / pharmacology
Dose-Response Relationship, Drug
Electrophoresis, Polyacrylamide Gel / methods
Enzyme Inhibitors / pharmacology
Excitatory Amino Acid Antagonists / pharmacology
Fluorescent Antibody Technique / methods
Hippocampus / cytology*
Kainic Acid / pharmacology
Mitogen-Activated Protein Kinase Kinases / metabolism
Nerve Growth Factors / chemistry, metabolism*
Neurons / drug effects, metabolism*
Pentylenetetrazole / pharmacology
Peptide Fragments / immunology, metabolism
Phosphorylation / drug effects
Receptors, N-Methyl-D-Aspartate / metabolism*
Seizures / chemically induced, metabolism*
Serine / metabolism*
|0/Carrier Proteins; 0/Enzyme Inhibitors; 0/Excitatory Amino Acid Antagonists; 0/Membrane Proteins; 0/Nerve Growth Factors; 0/Peptide Fragments; 0/Receptors, N-Methyl-D-Aspartate; 0/Stmn2 protein, rat; 487-79-6/Kainic Acid; 54-95-5/Pentylenetetrazole; 56-45-1/Serine; 77086-22-7/Dizocilpine Maleate; EC 18.104.22.168/Mitogen-Activated Protein Kinase Kinases|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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