| Site-specific incorporation of unnatural amino acids into proteins in Mammalian cells. | |
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MedLine Citation:
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PMID: 21956565 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Expanding the repertoire of genetically encoded amino acids in cultured mammalian cells requires the expression of the bacterial or archaeal pair of a tRNA and an aminoacyl-tRNA synthetase variant engineered to be specific to the amino acid, along with the supplementation of an unnatural amino acid in the growth medium. The expression of the pair is generally achieved by transfecting the cultured cells with the plasmids bearing the genes encoding the exogenous pair of translation molecules. Here, we provide a description of some of these plasmids and protocols for transfecting cells with the plasmids and preparing growth media supplemented with unnatural amino acids, to facilitate their incorporation into proteins at specific sites. |
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Authors:
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Nobumasa Hino; Kensaku Sakamoto; Shigeyuki Yokoyama |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 794 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2012 |
Date Detail:
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Created Date: 2011-09-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 215-28 Citation Subset: IM |
Affiliation:
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RIKEN Systems and Structural Biology Center, Tsurumi, Yokohama, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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