Document Detail


Site-specific gene integration in rice.
MedLine Citation:
PMID:  23135846     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Site-specific gene integration is a powerful technique for ensuring stable transgene expression. Transgenic plants produced by conventional transformation techniques often display highly variable transgene expression, which is mostly attributed to integration patterns consisting of multiple copies of transgene constructs. Therefore, it is desirable to generate single-copy integrations, preferably in a characterized genomic position. Precise integration of foreign genes into a selected genomic position can be obtained by employing site-specific recombination systems derived from bacteria or yeast. P1 bacteriophage Cre-lox system has been particularly successful in directing precise integration of foreign genes into "previously engineered" genomic sites. The resulting transgenic plants display stable expression through successive generations. Therefore, site-specific integration approach is useful for streamlining production of transgenic plants.
Authors:
Vibha Srivastava
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  956     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  83-93     Citation Subset:  IM    
Affiliation:
Department of Crop, Soil & Environmental Sciences and Department of Horticulture, University of Arkansas, Fayetteville, AR, USA, vibhas@uark.edu.
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