| Site-specific endonucleases RspLKI and RspLKII from Rhodococcus species LK2 are isoschizomers of SphI and BamHI. | |
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MedLine Citation:
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PMID: 9360299 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Two site-specific endonucleases, RspLKI and RspLKII, have been isolated and purified to functional homogeneity from the soil bacterium Rhodococcus species LK2. RspLKI recognizes the 5'-GCATG decreases C-3' DNA sequence and RspLKII recognizes the 5'-G decreases GATCC-3' sequence (arrows indicate DNA cleavage sites). The isolated enzymes are class II site specific endonucleases and are isoschizomers of endonucleases SphI and BamHI, respectively. |
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Authors:
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E V Zabaznaya; L A Zheleznaya; N I Matvienko |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemistry. Biokhimii͡a Volume: 62 ISSN: 0006-2979 ISO Abbreviation: Biochemistry Mosc. Publication Date: 1997 Aug |
Date Detail:
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Created Date: 1998-01-30 Completed Date: 1998-01-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376536 Medline TA: Biochemistry (Mosc) Country: RUSSIA |
Other Details:
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Languages: eng Pagination: 872-82 Citation Subset: IM |
Affiliation:
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Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, Russia. protres@sovam.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Ion Exchange DNA / metabolism Deoxyribonuclease BamHI / isolation & purification*, metabolism Deoxyribonucleases, Type II Site-Specific / isolation & purification*, metabolism Electrophoresis, Agar Gel Rhodococcus equi / enzymology* Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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9007-49-2/DNA; EC 3.1.21.-/Deoxyribonuclease BamHI; EC 3.1.21.-/endodeoxyribonuclease PaeI; EC 3.1.21.4/Deoxyribonucleases, Type II Site-Specific |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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