Document Detail

Site-specific endonucleases RspLKI and RspLKII from Rhodococcus species LK2 are isoschizomers of SphI and BamHI.
MedLine Citation:
PMID:  9360299     Owner:  NLM     Status:  MEDLINE    
Two site-specific endonucleases, RspLKI and RspLKII, have been isolated and purified to functional homogeneity from the soil bacterium Rhodococcus species LK2. RspLKI recognizes the 5'-GCATG decreases C-3' DNA sequence and RspLKII recognizes the 5'-G decreases GATCC-3' sequence (arrows indicate DNA cleavage sites). The isolated enzymes are class II site specific endonucleases and are isoschizomers of endonucleases SphI and BamHI, respectively.
E V Zabaznaya; L A Zheleznaya; N I Matvienko
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemistry. Biokhimii͡a     Volume:  62     ISSN:  0006-2979     ISO Abbreviation:  Biochemistry Mosc.     Publication Date:  1997 Aug 
Date Detail:
Created Date:  1998-01-30     Completed Date:  1998-01-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376536     Medline TA:  Biochemistry (Mosc)     Country:  RUSSIA    
Other Details:
Languages:  eng     Pagination:  872-82     Citation Subset:  IM    
Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
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MeSH Terms
Chromatography, Ion Exchange
DNA / metabolism
Deoxyribonuclease BamHI / isolation & purification*,  metabolism
Deoxyribonucleases, Type II Site-Specific / isolation & purification*,  metabolism
Electrophoresis, Agar Gel
Rhodococcus equi / enzymology*
Substrate Specificity
Reg. No./Substance:
9007-49-2/DNA; EC 3.1.21.-/Deoxyribonuclease BamHI; EC 3.1.21.-/endodeoxyribonuclease PaeI; EC, Type II Site-Specific

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