Document Detail


Site-specific drug delivery to pilosebaceous structures using polymeric microspheres.
MedLine Citation:
PMID:  8302759     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to improve the therapeutic index of adapalene, a new drug under development for the treatment of acne, site-specific delivery to the hair follicles using 50:50 poly(DL-lactic-co-glycolic acid) microspheres as particulate carriers was investigated in vitro and in vivo. The percutaneous penetration pathway of the microspheres was shown to be dependent on their mean diameter. Thus, after topical application onto hairless rat or human skin, adapalene-loaded microspheres (5-microns diameter) were specifically targeted to the follicular ducts and did not penetrate via the stratum corneum. The in vitro release of adapalene from the microspheres into artificial sebum at 37 degrees C was controlled and faster than the in vivo sebum excretion in humans. Aiming to reduce either the applied dose of drug or the frequency of administration, different formulations of adapalene-loaded microspheres were evaluated in vivo in the rhino mouse model. A dose-related comedolytic activity of topical formulations of adapalene-loaded microspheres was observed in this model. Furthermore, by applying a site-specific drug delivery system (0.1% adapalene) every other day or by administering a 10-fold less concentrated targeted formulation (0.01%) every day, a pharmacological activity equivalent to a daily application of an aqueous gel containing drug crystals (0.1% adapalene) was observed. Since an aqueous gel containing 10% adapalene-loaded microspheres was not irritating in a rabbit skin irritancy test, this formulation was applied onto forearms of human volunteers. Site-specific drug delivery was further evidenced by follicular biopsy. Since an aqueous gel containing 10% adapalene-loaded microspheres was not irritating in a rabbit skin irritancy test, this formulation was applied onto forearms of human volunteers. Site-specific drug delivery was further evidenced by follicular biopsy.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
A Rolland; N Wagner; A Chatelus; B Shroot; H Schaefer
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Pharmaceutical research     Volume:  10     ISSN:  0724-8741     ISO Abbreviation:  Pharm. Res.     Publication Date:  1993 Dec 
Date Detail:
Created Date:  1994-03-04     Completed Date:  1994-03-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8406521     Medline TA:  Pharm Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1738-44     Citation Subset:  IM    
Affiliation:
Cird Galderma, Sophia Antipolis, Valbonne, France.
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MeSH Terms
Descriptor/Qualifier:
Administration, Topical
Animals
Dermatologic Agents / administration & dosage,  adverse effects,  pharmacokinetics*
Drug Carriers
Female
Hair / metabolism*
Humans
Irritants / adverse effects
Lactic Acid*
Male
Mice
Microspheres
Naphthalenes / administration & dosage,  adverse effects,  pharmacokinetics*
Polyglycolic Acid*
Polymers
Rabbits
Rats
Sebaceous Glands / metabolism*
Skin / drug effects
Skin Absorption / drug effects
Chemical
Reg. No./Substance:
0/Dermatologic Agents; 0/Drug Carriers; 0/Irritants; 0/Naphthalenes; 0/Polymers; 0/polylactic acid-polyglycolic acid copolymer; 106685-40-9/adapalene; 26009-03-0/Polyglycolic Acid; 50-21-5/Lactic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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