Document Detail


Site-specific cleavage of human chromosome 4 mediated by triple-helix formation.
MedLine Citation:
PMID:  1836279     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Direct physical isolation of specific DNA segments from the human genome is a necessary goal in human genetics. For testing whether triple-helix mediated enzymatic cleavage can liberate a specific segment of a human chromosome, the tip of human chromosome 4, which contains the entire candidate region for the Huntington's disease gene, was chosen as a target. A 16-base pyrimidine oligodeoxyribonucleotide was able to locate a 16-base pair purine target site within more than 10 gigabase pairs of genomic DNA and mediate the exact enzymatic cleavage at that site in more than 80 percent yield. The recognition motif is sufficiently generalizable that most cosmids should contain a sequence targetable by triple-helix formation. This method may facilitate the orchestrated dissection of human chromosomes from normal and affected individuals into megabase sized fragments and facilitate the isolation of candidate gene loci.
Authors:
S A Strobel; L A Doucette-Stamm; L Riba; D E Housman; P B Dervan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  254     ISSN:  0036-8075     ISO Abbreviation:  Science     Publication Date:  1991 Dec 
Date Detail:
Created Date:  1992-01-21     Completed Date:  1992-01-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1639-42     Citation Subset:  IM    
Affiliation:
Arnold and Mabel Beckman Laboratories of Chemical Synthesis, California Institute of Technology, Pasadena 91125.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Chromosome Mapping / methods
Chromosomes, Human, Pair 4 / ultrastructure*
DNA Damage
Humans
Huntington Disease / genetics
Hydrogen Bonding
Molecular Sequence Data
Nucleic Acid Conformation
Oligodeoxyribonucleotides / chemistry
Restriction Mapping
Grant Support
ID/Acronym/Agency:
HG00098/HG/NHGRI NIH HHS; HG00329/HG/NHGRI NIH HHS
Chemical
Reg. No./Substance:
0/Oligodeoxyribonucleotides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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