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Site-specific cleavage of chromosomes in vitro through Cre-lox recombination.
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MedLine Citation:
PMID:  7596819     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Site-specific recombination systems are useful tools for chromosome engineering in vivo and site-specific DNA cleavage methods have applications in genome analysis and gene isolation. Here, we report a new method to fragment chromosomes in vitro using the Cre-lox site-specific recombination system. Two lox sites were targeted into the 5.7 Mb chromosomes I of Schizosaccharomyces pombe. In vitro recombination between chromosomal lox sites and exogenously provided lox oligonucleotides 'cleaved' the chromosome at the defined lox sequences. Site-specific cleavage of lox sites in the tobacco genome was also demonstrated. This recombination-based cleavage method provides a novel approach for structural and functional analyses of eukaryotic chromosomes as it allows direct isolation of chromosome regions that correspond to phenotypes revealed through Cre-lox mediated chromosome rearrangements in vivo. Moreover, recombination with end-labeled lox oligonucleotides would permit the specific end-labeling of chromosome segments to facilitate the long range mapping of chromosomes.
Authors:
M Qin; E Lee; T Zankel; D W Ow
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Nucleic acids research     Volume:  23     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1995 Jun 
Date Detail:
Created Date:  1995-08-03     Completed Date:  1995-08-03     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1923-7     Citation Subset:  IM    
Affiliation:
Plant Gene Expression Center, US Department of Agriculture, Albany, CA 94710, USA.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Chromosomes / genetics*
Molecular Sequence Data
Plasmids
Recombination, Genetic
Schizosaccharomyces / genetics*
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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Print publication date: Day: 11 Month: 6 Year: 1995
Volume: 23 Issue: 11
First Page: 1923 Last Page: 1927
ID: 306964
PubMed Id: 7596819
Publisher Item Identifier: 5t0102

Site-specific cleavage of chromosomes in vitro through Cre-lox recombination.
M Qin
E Lee
T Zankel
D W Ow
Plant Gene Expression Center, US Department of Agriculture, Albany, CA 94710, USA.



Article Categories:
  • Research Article


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