Document Detail


Sister-chromatid exchanges (SCEs), cell survival and mutation in HeLa s3 cells with different sensitivity to alkylating agents; evidence that SCE induction and cell survival or mutation induction are dissociable.
MedLine Citation:
PMID:  2811915     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We previously isolated N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-resistant cells, MR from HeLa S3 Mer- cells. In the present study, we have isolated 1-(4-amino-2-methyl-5-pyrimidinyl)methyl-3-(2-chloroethyl)-3-nitrosourea (ACNU)-resistant cells, ACr. The MR cells had only a little O6-methylguanine-DNA methyltransferase (MT) activity, while the ACr cells had increased MT activity and also became resistant to the cytotoxic effect of MNNG. We compared the induction of sister-chromatid exchanges (SCEs), cell survival and mutation in these HeLa S3 cells with different sensitivity to MNNG. The ACr cells were much more resistant than the parental HeLa S3 Mer- cells to cytotoxicity, mutagenicity and SCE induction by MNNG, showing a positive correlation between SCE induction and cell killing or mutation. In contrast, this positive relationship was not observed between HeLa S3 Mer- and MR cells. These results suggest that O6-methylguanine (O6-MeG) is involved in the induction of the biological effects of MNNG such as cytotoxicity, mutagenicity and SCEs, and also indicate that SCE induction does not always correlate with cell killing and mutation.
Authors:
R Ishida; K R Utsumi; T Takahashi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Mutation research     Volume:  215     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1989 Nov 
Date Detail:
Created Date:  1989-12-21     Completed Date:  1989-12-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  69-77     Citation Subset:  IM    
Affiliation:
Laboratory of Biochemistry, Aichi Cancer Center Research Institute, Nagoya, Japan.
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MeSH Terms
Descriptor/Qualifier:
Alkylating Agents / pharmacology*
Cell Survival / drug effects*
Hela Cells / cytology,  drug effects
Humans
Lomustine / pharmacology
Methylnitronitrosoguanidine / pharmacology
Mutation*
Nimustine / pharmacology
Sister Chromatid Exchange / drug effects*
Structure-Activity Relationship
Chemical
Reg. No./Substance:
0/Alkylating Agents; 13010-47-4/Lomustine; 42471-28-3/Nimustine; 70-25-7/Methylnitronitrosoguanidine

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