Document Detail


Sister chromatid exchange distributions in rabbit lymphocytes treated with streptonigrin.
MedLine Citation:
PMID:  6653504     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Streptonigrin (NSC-45383), a direct-acting clastogen which induces SCEs in vivo and chromosome aberrations both in vivo and in vitro, was evaluated for SCE induction in both G0 and stimulated rabbit lymphocytes. Determinations were made for 16 cultures from seven female rabbits. These included controls as well as cells exposed to 90 micrograms/kg in vivo, cells pulse-treated with 50 ng/ml in vitro, and a culture continuously exposed to 5 ng/ml in vitro. For all cultures the SCE/cell frequency was determined from 20 complete (44 chromosome) metaphases and, in selected cultures, SCEs on individual chromosomes (880 per culture from 20 cells) were enumerated to determine SCE/chromosome frequency and the chromosomal distribution of SCEs. Analysis of variance and least significant difference tests of the square root x transformed SCE/cell data show that cells exposed to streptonigrin while dividing have significantly higher (P less than 0.01) frequencies (over double the control 5.3 SCE/cell value) whereas treated G0 cells were not significantly different from the controls. Dispersion analysis of both SCE/cell and SCE/chromosome data confirms the adequacy of the Poisson distribution for spontaneous or baseline but not streptonigrin-induced SCEs.
Authors:
R J DuFrain
Related Documents :
9118914 - Interphase cytogenetics of workers exposed to benzene.
1176134 - The human leukocyte test system. vi. the use of sister chromatid exchanges as possible ...
7747744 - Possible genotoxicity in low level benzene exposure.
864784 - Cytogenetic investigations on lymphocytes from workers exposed to vinyl chloride.
17188484 - The 1.6mb chromosome carrying the avirulence gene avrpik in magnaporthe oryzae isolate ...
18832704 - Uncoupling the roles of hla-drb1 and hla-drb5 genes in multiple sclerosis.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Environmental mutagenesis     Volume:  5     ISSN:  0192-2521     ISO Abbreviation:  Environ Mutagen     Publication Date:  1983  
Date Detail:
Created Date:  1984-02-14     Completed Date:  1984-02-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7909737     Medline TA:  Environ Mutagen     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  813-24     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Crossing Over, Genetic / drug effects*
Female
Lymphocytes / ultrastructure*
Probability
Rabbits
Sister Chromatid Exchange / drug effects*
Streptonigrin / toxicity*
Grant Support
ID/Acronym/Agency:
HD 08828/HD/NICHD NIH HHS; HD 11709/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
3930-19-6/Streptonigrin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The induction of bacterial mutation and hepatocyte unscheduled DNA synthesis by monosubstituted anil...
Next Document:  Further study on the cytogenetic effects of combined chemotherapy with isoniazid and para-aminosalic...