Document Detail

Sister chromatid exchange distributions in rabbit lymphocytes treated with streptonigrin.
MedLine Citation:
PMID:  6653504     Owner:  NLM     Status:  MEDLINE    
Streptonigrin (NSC-45383), a direct-acting clastogen which induces SCEs in vivo and chromosome aberrations both in vivo and in vitro, was evaluated for SCE induction in both G0 and stimulated rabbit lymphocytes. Determinations were made for 16 cultures from seven female rabbits. These included controls as well as cells exposed to 90 micrograms/kg in vivo, cells pulse-treated with 50 ng/ml in vitro, and a culture continuously exposed to 5 ng/ml in vitro. For all cultures the SCE/cell frequency was determined from 20 complete (44 chromosome) metaphases and, in selected cultures, SCEs on individual chromosomes (880 per culture from 20 cells) were enumerated to determine SCE/chromosome frequency and the chromosomal distribution of SCEs. Analysis of variance and least significant difference tests of the square root x transformed SCE/cell data show that cells exposed to streptonigrin while dividing have significantly higher (P less than 0.01) frequencies (over double the control 5.3 SCE/cell value) whereas treated G0 cells were not significantly different from the controls. Dispersion analysis of both SCE/cell and SCE/chromosome data confirms the adequacy of the Poisson distribution for spontaneous or baseline but not streptonigrin-induced SCEs.
R J DuFrain
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Environmental mutagenesis     Volume:  5     ISSN:  0192-2521     ISO Abbreviation:  Environ Mutagen     Publication Date:  1983  
Date Detail:
Created Date:  1984-02-14     Completed Date:  1984-02-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7909737     Medline TA:  Environ Mutagen     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  813-24     Citation Subset:  IM    
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MeSH Terms
Crossing Over, Genetic / drug effects*
Lymphocytes / ultrastructure*
Sister Chromatid Exchange / drug effects*
Streptonigrin / toxicity*
Grant Support
Reg. No./Substance:

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