Document Detail


Sister chromatid differentiation and isolabeling of chromosomes.
MedLine Citation:
PMID:  6165669     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Isolabeling observed during sister chromatid differentiation (SCD) was studied from human skin fibroblasts by the fluorescence-plus-Giemsa (FPG) technique. Bromodeoxyuridine (BrdU) was fed to exponentially dividing cells for 52 h to enable completion of two consecutive cycles of DNA replication. During this period, the late-replicating regions of some chromosomes were able to go through three replication cycles. These chromosome regions had evidently incorporated BrdU bifiliarly in both chromatids and hence, on staining with FPG, appeared isostained (isolabeled). Thus, incubation of exponentially dividing cells with BrdU for a period longer than that required for two cell cycles appears to be a suitable method for revealing the late-replicating regions of the genome, such as the X chromosome in a human female, as isolabeled. In another experiment with Indian muntjac chromosomes, isolabeled segments were darkly stained, which suggested unifilar incorporation of BrdU. In this case, unequal crossing-over or an unequal distribution of thymine residues probably is responsible for the isolabel.
Authors:
S Ved Brat; R S Verma; H Dosik
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Human genetics     Volume:  56     ISSN:  0340-6717     ISO Abbreviation:  Hum. Genet.     Publication Date:  1981  
Date Detail:
Created Date:  1981-08-20     Completed Date:  1981-08-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7613873     Medline TA:  Hum Genet     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  305-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Bromodeoxyuridine / pharmacology
Cells, Cultured
Chromatids / ultrastructure*
Crossing Over, Genetic*
DNA Replication
Deer / genetics
Humans
Sister Chromatid Exchange*
Skin / ultrastructure
Staining and Labeling
Grant Support
ID/Acronym/Agency:
1-CP-43251/CP/NCI NIH HHS
Chemical
Reg. No./Substance:
59-14-3/Bromodeoxyuridine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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