| Sirolimus inhibits growth of human hepatoma cells alone or combined with tacrolimus, while tacrolimus promotes cell growth. | |
| | |
MedLine Citation:
|
PMID: 15770715 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
AIM: Standard immunosuppression after organ transplantation stimulates tumor growth. Sirolimus has a strong antiproliferative and a tumor inhibiting effect. The purpose is to assess the effect on tumor growth of the immuno-suppressive compounds sirolimus and tacrolimus alone and in combination on cells of human hepatocellular carcinoma. METHODS: We used the human cell lines SK-Hep 1 and Hep 3B derived from hepatocellular carcinoma. Proliferation analyses after treatment with sirolimus, tacrolimus, or the combination of both were performed. FACS analyses were done to reveal cell cycle changes and apoptotic cell death. The expression of apoptosis-related proteins was estimated by Western blots. RESULTS: Sirolimus alone or combined with tacrolimus inhibited the growth of both cell lines after 5 d by up to 35% in SK-Hep 1 cells, and by up to 68% in Hep 3B cells at 25 ng/mL. Tacrolimus alone stimulated the growth by 12% after 5 ng/mL and by 25% after 25 ng/mL in Hep 3B cells. We found an increase of apoptotic Hep 3B cells from 6 to 16%, and a G1-arrest in SK-Hep 1 cells with an increase of cells from 61 to 82%, when sirolimus and tacrolimus were combined. Bcl-2 was down-regulated in Hep 3B, but not in SK-Hep 1 cells after combined treatment. CONCLUSION: Sirolimus appears to inhibit the growth of hepatocellular carcinoma cells alone and in combination with tacrolimus. Sirolimus seems to inhibit the growth stimulation of tacrolimus. |
| | |
Authors:
|
Guido Schumacher; Marijke Oidtmann; Anne Rueggeberg; Dietmar Jacob; Sven Jonas; Jan-M Langrehr; Ruth Neuhaus; Marcus Bahra; Peter Neuhaus |
Related Documents
:
|
16003725 - Cisplatin-dependent upregulation of death receptors 4 and 5 augments induction of apopt... 12811515 - The topoisomerase i inhibitor topotecan increases the sensitivity of prostate tumor cel... 14981905 - Inducible expression of rbap46 activates c-jun nh2-terminal kinase-dependent apoptosis ... 9973225 - Chemotherapy augments trail-induced apoptosis in breast cell lines. 10783465 - Proto-metastatic core and comet effect: new theoretical model of the mechanism of metas... 9989495 - Bcl10 is involved in t(1;14)(p22;q32) of malt b cell lymphoma and mutated in multiple t... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: World journal of gastroenterology : WJG Volume: 11 ISSN: 1007-9327 ISO Abbreviation: World J. Gastroenterol. Publication Date: 2005 Mar |
Date Detail:
|
Created Date: 2005-03-16 Completed Date: 2005-04-19 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100883448 Medline TA: World J Gastroenterol Country: China |
Other Details:
|
Languages: eng Pagination: 1420-5 Citation Subset: IM |
Affiliation:
|
Department of General, Visceral, and Transplantation Surgery, Charite Campus Virchow Klinikum, Humboldt University, Augustenburger Platz 1, Berlin 13353, Germany. guido.schumacher@charite.de |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Apoptosis
/
drug effects Carcinoma, Hepatocellular / pathology*, physiopathology Cell Division / drug effects Cell Line, Tumor Drug Combinations G1 Phase Humans Immunosuppressive Agents / pharmacology* Liver Neoplasms / pathology*, physiopathology Sirolimus / pharmacology* Tacrolimus / pharmacology* |
| Chemical | |
Reg. No./Substance:
|
0/Drug Combinations; 0/Immunosuppressive Agents; 109581-93-3/Tacrolimus; 53123-88-9/Sirolimus |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Co-administration of cyclosporine an alleviates thioacetamide-induced liver injury.
Next Document: Comparison between combination therapy of percutaneous ethanol injection and radiofrequency ablation...