| Sinorhizobium meliloti CpdR1 is critical for co-ordinating cell cycle progression and the symbiotic chronic infection. | |
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MedLine Citation:
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PMID: 19602145 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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ATP-driven proteolysis plays a major role in regulating the bacterial cell cycle, development and stress responses. In the nitro -fixing symbiosis with host plants, Sinorhizobium meliloti undergoes a profound cellular differentiation, including endoreduplication of the ome. The regulatory mechanisms governing the alterations of the S. meliloti cell cycle in planta are largely unknown. Here, we report the characterization of two cpdR homologues, cpdR1 and cpdR2, of S. meliloti that encode single-domain response regulators. In Caulobacter crescentus, CpdR controls the polar localization of the ClpXP protease, thereby mediating the regulated proteolysis of key protein(s), such as CtrA, involved in cell cycle progression. The S. meliloti cpdR1-null mutant can invade the host cytoplasm, however, the intracellular bacteria are unable to differentiate into bacteroids. We show that S. meliloti CpdR1 has a polar localization pattern and a role in ClpX positioning similar to C. crescentus CpdR, suggesting a conserved function of CpdR proteins among alpha-proteobacteria. However, in S. meliloti, free-living cells of the cpdR1-null mutant show a striking morphology of irregular coccoids and aberrant DNA replication. Thus, we demonstrate that CpdR1 mediates the co-ordination of cell cycle events, which are critical for both the free-living cell division and the differentiation required for the chronic intracellular infection. |
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Authors:
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Hajime Kobayashi; Nicole J De Nisco; Peter Chien; Lyle A Simmons; Graham C Walker |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2009-07-07 |
Journal Detail:
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Title: Molecular microbiology Volume: 73 ISSN: 1365-2958 ISO Abbreviation: Mol. Microbiol. Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-08-19 Completed Date: 2009-10-13 Revised Date: 2010-12-03 |
Medline Journal Info:
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Nlm Unique ID: 8712028 Medline TA: Mol Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 586-600 Citation Subset: IM |
Affiliation:
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Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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genetics,
metabolism* Cell Cycle* DNA Replication DNA, Bacterial / biosynthesis Gene Expression Profiling Gene Expression Regulation, Bacterial Mutagenesis, Insertional Sinorhizobium meliloti / cytology*, genetics, metabolism Symbiosis* |
| Grant Support | |
ID/Acronym/Agency:
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5K99GM084157-01/GM/NIGMS NIH HHS; CA21615-27/CA/NCI NIH HHS; GM31010/GM/NIGMS NIH HHS; P30 ES002109/ES/NIEHS NIH HHS; R01 CA021615-27/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/DNA, Bacterial |
| Comments/Corrections | |
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