Document Detail


Singlet oxygen quenching activity of human serum.
MedLine Citation:
PMID:  15720827     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Singlet oxygen is regarded as contributing to the pathogenesis of various diseases including light-induced skin disorders and inflammatory response. In this study, the correlation between singlet oxygen quenching activity (SOQA) of human serum and blood biochemistry or life-style was evaluated. Healthy volunteers were recruited and carried out a measurement of SOQA by using electron paramagnetic resonance (EPR) and a questionnaire survey about a smoking. It was demonstrated that major quenchers of singlet oxygen in serum are proteins, and small molecular anti-oxidants relatively play a minor role. SOQA of whole sera showed no correlation with protein concentration, but positively correlated with SOQA of small molecular fraction. In vitro studies demonstrated that the decrease of sulfhydryl groups by NO or superoxide significantly attenuated SOQA of albumin. Together, these results may imply that the underlying oxidative condition in each individual influences both small molecular antioxidant states and the sulfhydryl content of serum proteins. SOQA of sera from women with a smoking history was significantly lower compared to non-smoking women, suggesting that the smoking habit impaired the defense mechanism against singlet oxygen.
Authors:
Tatsuya Kon; Toru Tanigawa; Kohsuke Hayamizu; Manzhen Shen; Tomoko Tsuji; Yuji Naito; Toshikazu Yoshikawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Redox report : communications in free radical research     Volume:  9     ISSN:  1351-0002     ISO Abbreviation:  Redox Rep.     Publication Date:  2004  
Date Detail:
Created Date:  2005-02-21     Completed Date:  2005-07-19     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  9511366     Medline TA:  Redox Rep     Country:  England    
Other Details:
Languages:  eng     Pagination:  325-30     Citation Subset:  IM    
Affiliation:
Central Research Laboratory, FANCL Corporation, Kanagawa, Yokohama, Japan. kon@fancl.co.jp
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Blood Proteins / analysis,  physiology*
Electron Spin Resonance Spectroscopy
Female
Humans
Life Style
Male
Middle Aged
Oxidation-Reduction
Oxidative Stress
Serum*
Serum Albumin / analysis
Singlet Oxygen / blood*,  physiology*
Smoking / blood
Sulfhydryl Compounds / blood
gamma-Globulins / analysis
Chemical
Reg. No./Substance:
0/Blood Proteins; 0/Serum Albumin; 0/Sulfhydryl Compounds; 0/gamma-Globulins; 17778-80-2/Singlet Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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