| Singlet oxygen-induced apoptosis of cancer cells using upconversion fluorescent nanoparticles as a carrier of photosensitizer. | |
| | |
MedLine Citation:
|
PMID: 20044035 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The photodynamic effect of upconversion nanoparticles loaded with a photosensitizer was studied on murine bladder cancer cells (MB49). Mesoporous silica was coated onto sodium yttrium fluoride upconversion nanocrystals to form a core-shell structure and then loaded with the photosensitizer zinc (II)-phthalocyanine into the porous silica. The nanoparticles displayed a uniform spherical shape with an average diameter of about 50 nm and showed good dispersibility in water. Intracellular uptake study in MB49 cells revealed a time- and concentration-dependent accumulation of these nanoparticles. Upon irradiation with 980-nm near-infrared light, their efficiency in activating the loaded zinc (II)-phthalocyanine to generate singlet oxygen molecules was confirmed in live cells. The cytotoxic effect of the released singlet oxygen from the nanoplatform was proven by cell viability assay, confocal microscopy, DNA agarose gel electrophoresis, cytochrome c-releasing assay, and prostate-specific antigen-enzyme-linked immunosorbent assay, all of which showed a strong photodynamic effect of the nanoparticles on MB49 cells. This suggests the efficacy of sodium yttrium fluoride upconversion nanoparticles as a carrier for photosensitizers and their use in photodynamic therapy of cancer and some other diseases. FROM THE CLINICAL EDITOR: In this study, the photodynamic effect of upconversion nanoparticles loaded with a photosensitizer was investigated on murine bladder cancer cells, with strongly positive results, which may pave its way to future clinical use in malignant tumors and potentially other diseases. |
| | |
Authors:
|
Huichen Guo; Haisheng Qian; Niagara Muhammad Idris; Yong Zhang |
Related Documents
:
|
2536345 - Stability and reactivation of tyrosine radicals from ribonucleotide reductase in tumor ... 18297605 - New method for the detection of reactive oxygen species in anti-tumoural activity of ad... 9576845 - Rac1, and not rac2, is involved in the regulation of the intracellular hydrogen peroxid... 11539395 - Consumption, supply and transport: self-organization without direct communication. 21945045 - Toxic effects following phosgene exposure of human epithelial lung cells in vitro using... 17030625 - Auf1 cell cycle variations define genomic dna methylation by regulation of dnmt1 mrna s... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-01-04 |
Journal Detail:
|
Title: Nanomedicine : nanotechnology, biology, and medicine Volume: 6 ISSN: 1549-9642 ISO Abbreviation: Nanomedicine Publication Date: 2010 Jun |
Date Detail:
|
Created Date: 2010-06-01 Completed Date: 2010-09-27 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101233142 Medline TA: Nanomedicine Country: United States |
Other Details:
|
Languages: eng Pagination: 486-95 Citation Subset: IM |
Affiliation:
|
Division of Bioengineering, Faculty of Engineering, National University of Singapore, Singapore, Singapore. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Apoptosis
/
drug effects* Cell Line, Tumor Cell Nucleus / drug effects, metabolism Cell Nucleus Shape / drug effects Cell Survival / drug effects Cytochromes c / secretion DNA Fragmentation / drug effects Drug Carriers / chemistry* Fluorides Humans Indoles / metabolism* Intracellular Space / drug effects, metabolism Male Nanomedicine / methods Nanoparticles / chemistry*, ultrastructure Neoplasms / pathology* Organometallic Compounds / metabolism* Oxidative Stress / drug effects Photochemotherapy Photosensitizing Agents / therapeutic use* Porosity / drug effects Prostate-Specific Antigen / metabolism Silicon Dioxide / chemistry Singlet Oxygen / pharmacology* Spectrometry, Fluorescence Yttrium |
| Chemical | |
Reg. No./Substance:
|
0/Drug Carriers; 0/Fluorides; 0/Indoles; 0/Organometallic Compounds; 0/Photosensitizing Agents; 0/sodium yttriumtetrafluoride; 14320-04-8/Zn(II)-phthalocyanine; 17778-80-2/Singlet Oxygen; 7440-65-5/Yttrium; 7631-86-9/Silicon Dioxide; 9007-43-6/Cytochromes c; EC 3.4.21.77/Prostate-Specific Antigen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Release Dynamics of Ciprofloxacin (CFx) From Swellable Nanocarriers of Poly-(2-Hydroxyethyl methacry...
Next Document: FGFR3 Expression in Xenopus laevis.