| Singlet Oxygen Sensor Green(®) : Photochemical Behavior in Solution and in a Mammalian Cell. | |
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MedLine Citation:
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PMID: 21272007 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The development of efficient and selective luminescent probes for reactive oxygen species, particularly for singlet molecular oxygen, is currently of great importance. In this study, the photochemical behavior of Singlet Oxygen Sensor Green(®) (SOSG), a commercially available fluorescent probe for singlet oxygen, was examined. Despite published claims to the contrary, the data presented herein indicate that SOSG can, in fact, be incorporated into a living mammalian cell. However, for a number of reasons, caution must be exercised when using SOSG. First, it is shown that the immediate product of the reaction between SOSG and singlet oxygen is, itself, an efficient singlet oxygen photosensitizer. Second, SOSG appears to efficiently bind to proteins which, in turn, can influence uptake by a cell as well as behavior in the cell. As such, incorrect use of SOSG can yield misleading data on yields of photosensitized singlet oxygen production, and can also lead to photooxygenation-dependent adverse effects in the system being investigated. |
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Authors:
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Anita Gollmer; Jacob Arnbjerg; Frances H Blaikie; Brian Wett Pedersen; Thomas Breitenbach; Kim Daasbjerg; Marianne Glasius; Peter R Ogilby |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-1-27 |
Journal Detail:
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Title: Photochemistry and photobiology Volume: - ISSN: 1751-1097 ISO Abbreviation: - Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-1-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376425 Medline TA: Photochem Photobiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Journal compilation © 2011 American Society for Photobiology. |
Affiliation:
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Center for Oxygen Microscopy and Imaging (COMI), Department of Chemistry, Aarhus University, Langelandsgade 140, 8000 Århus C, Denmark. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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