Document Detail


Singlepath Salmonella. Performance-Tested Method 060401.
MedLine Citation:
PMID:  16640289     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Singlepath Salmonella is an immunochromatographic (lateral flow) assay for the presumptive qualitative detection of Salmonella spp. in food. The AOAC Performance-Tested Method study evaluated Singlepath Salmonella as an effective method for the detection of Salmonella spp. in the following selected foods: dried skimmed milk, black pepper, dried pet food, desiccated coconut, cooked peeled frozen prawns, raw ground beef, and raw ground turkey. When the foods were inoculated with Salmonella spp. at levels ranging from low [0.23-1.08 colony forming units (CFU)/25 g] to high (2.3-6.0 CFU/25 g), a Chi-square value of 0.9 indicated that there was no significant difference between Singlepath Salmonella and the ISO 6579:2002 reference method. Singlepath Salmonella gave a false-positive rate of 7.3% and a false-negative rate of 2.5%. For the inclusivity study, all 105 Salmonella serovars reacted with Singlepath Salmonella. For the exclusivity study, 58 non-Salmonella spp. were tested. There were no cross-reactions with Singlepath Salmonella from these strains.
Authors:
Lisa Thompson; Charlotte Lindhardt
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  89     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2006 Mar-Apr
Date Detail:
Created Date:  2006-04-27     Completed Date:  2006-05-23     Revised Date:  2008-03-17    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  417-32     Citation Subset:  IM    
Affiliation:
Merck Chemicals Ltd., Newport Technical Centre, 5 The Courtyard, Imperial Park, Newport NP10 8UL, Wales, United Kingdom. lisa.thompson@merckchem.co.uk
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MeSH Terms
Descriptor/Qualifier:
Bacteria / chemistry
Food Contamination / analysis*
Food Microbiology
Immunochemistry
Indicators and Reagents
Reagent Kits, Diagnostic*
Reference Standards
Reproducibility of Results
Salmonella / chemistry*
Seafood / microbiology
Chemical
Reg. No./Substance:
0/Indicators and Reagents; 0/Reagent Kits, Diagnostic

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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