| Single-unit in vivo recordings from the optic chiasm of rat. | |
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MedLine Citation:
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PMID: 20364119 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Information about the visual world is transmitted to the brain in sequences of action potentials in retinal ganglion cell axons that make up the optic nerve. In vivo recordings of ganglion cell spike trains in several animal models have revealed much of what is known about how the early visual system processes and encodes visual information. However, such recordings have been rare in one of the most common animal models, the rat, possibly owing to difficulty in detecting spikes fired by small diameter axons. The many retinal disease models involving rats motivate a need for characterizing the functional properties of ganglion cells without disturbing the eye, as with intraocular or in vitro recordings. Here, we demonstrate a method for recording ganglion cell spike trains from the optic chiasm of the anesthetized rat. We first show how to fabricate tungsten-in-glass electrodes that can pick up electrical activity from single ganglion cell axons in rat. The electrodes outperform all commercial ones that we have tried. We then illustrate our custom-designed stereotaxic system for in vivo visual neurophysiology experiments and our procedures for animal preparation and reliable and stable electrode placement in the optic chiasm. |
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Authors:
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Daniel K Freeman; Walter F Heine; Christopher L Passaglia |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Video-Audio Media Date: 2010-04-02 |
Journal Detail:
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Title: Journal of visualized experiments : JoVE Volume: - ISSN: 1940-087X ISO Abbreviation: J Vis Exp Publication Date: 2010 |
Date Detail:
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Created Date: 2010-04-05 Completed Date: 2010-04-19 Revised Date: 2012-04-02 |
Medline Journal Info:
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Nlm Unique ID: 101313252 Medline TA: J Vis Exp Country: United States |
Other Details:
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Languages: eng Pagination: - Citation Subset: IM |
Affiliation:
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Department of Biomedical Engineering, Boston University, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Action Potentials Animals Axons / physiology Electrodes Electrophysiology / instrumentation, methods* Glass Optic Chiasm / physiology* Rats Retinal Ganglion Cells / physiology Tungsten |
| Grant Support | |
ID/Acronym/Agency:
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R01-EY016849A/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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7440-33-7/Tungsten |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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