| Single and twin ongoing pregnancies in two cases of previous ART failure after ICSI performed with sperm sorted using annexin V microbeads. | |
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MedLine Citation:
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PMID: 20149363 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To treat couples with intracytoplasmic sperm injection (ICSI) after annexin V sperm sorting. DESIGN: Two case reports. SETTING: Department of Reproductive Medicine at a private medical institute. PATIENT(S): Couples on infertility treatment, donor oocytes. INTERVENTION(S): Sperm sorted with annexin V magnetic microbeads before ICSI, day 3 embryo transfer; case 1: ovum donation; case 2: patient oocytes. MAIN OUTCOME MEASURE(S): 1) Sperm DNA fragmentation (terminal deoxynucleotide transferase-mediated dUTP nick-end labeling [TUNEL]) and active caspase-3 (immunocytochemistry); 2) fertilization rate, embryonic quality, blastocyst development of nontransferred embryos, and pregnancy outcome after ICSI of sorted sperm. RESULT(S): Case 1: Premature ovarian failure patient with previous fertilization failures: asthenoteratozoospermia, abnormal DNA fragmentation (TUNEL 30%; normal <20%). ICSI with annexin V-treated sperm done on six donated metaphase II (MII) oocytes; four fertilized, and a 5-cell/grade-2 and a 6-cell/grade-2-3 embryo were transfered. A day 5 blastocyst was cryopreserved. The patient was in the last trimester of gestation. Case 2: Couple with >4 years of primary infertility and recent ICSI failure. Semen with teratozoospermia (5% normal forms [Kruger]) and abnormal active caspase-3 (16%; normal <11%). ICSI with annexin V-treated sperm done on 9 MII oocytes. All fertilized; a 7-cell/grade-1 and an 8-cell/grade-1-2 embryo were transferred. A day 5 expanded blastocyst was cryopreserved. The patient was in the second trimester of a twin normal pregnancy. CONCLUSION(S): Sperm sorting with annexin V columns was effective in the treatment of two cases of ICSI failure, resulting in a single and a twin pregnancy after transfer of two embryos in each case. |
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Authors:
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Ester Polak de Fried; Flavia Denaday |
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Publication Detail:
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Type: Case Reports; Journal Article Date: 2010-02-10 |
Journal Detail:
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Title: Fertility and sterility Volume: 94 ISSN: 1556-5653 ISO Abbreviation: Fertil. Steril. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-25 Completed Date: 2010-06-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372772 Medline TA: Fertil Steril Country: United States |
Other Details:
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Languages: eng Pagination: 351.e15-8 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010. Published by Elsevier Inc. |
Affiliation:
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Department of Reproductive Medicine, CER Medical Institute, School of Medicine, University of Buenos Aires, Buenos Aires, Argentina. ester_polak@cermed.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Annexin A5 / administration & dosage* Cell Separation / methods DNA Fragmentation Female Humans Male Microspheres* Pregnancy Pregnancy Outcome* Sperm Injections, Intracytoplasmic / methods* Spermatozoa / cytology*, drug effects Treatment Failure Twins |
| Chemical | |
Reg. No./Substance:
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0/Annexin A5 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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