| Single tube, six-color flow cytometric analysis is a sensitive and cost-effective technique for assaying clonal plasma cells. | |
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MedLine Citation:
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PMID: 20395515 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bone marrow flow cytometric analysis is a powerful and rapid tool for evaluating plasma cell myeloma. By using a noncontrolled patient population in various stages of diagnosis and treatment, we compared 6-color (single-tube) and 4-color (multiple-tube) flow cytometric immunophenotyping protocols. Prospective comparison in 52 cases demonstrated improved ability to detect clonal plasma cells or identical diagnoses in 100% of the cases using 6-color, single-tube analysis. In cases in which 6-color flow cytometric analysis improved detection of a clonal population, concurrent biopsy showed less than 5% involvement by plasma cell myeloma, suggesting that 6-color flow cytometry has an advantage in patients with a low disease burden. In addition, the simplification of the procedure resulted in substantial savings in technologist time and reagent costs. Taken together, this study demonstrates that 6-color flow cytometry is an excellent, cost-effective means to assay for clonal plasma cells in a noncontrolled patient population. |
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Authors:
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Derek K Marsee; Betty Li; David M Dorfman |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: American journal of clinical pathology Volume: 133 ISSN: 1943-7722 ISO Abbreviation: Am. J. Clin. Pathol. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-04-16 Completed Date: 2010-05-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370470 Medline TA: Am J Clin Pathol Country: United States |
Other Details:
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Languages: eng Pagination: 694-9 Citation Subset: AIM; IM |
Affiliation:
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Dept of Pathology, Brigham and Women's Hospital, 75 Francis St, Boston, MA 02115, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bone Marrow Cells
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pathology Clone Cells / immunology, pathology* Cost-Benefit Analysis Flow Cytometry* / economics, instrumentation, methods Humans Immunophenotyping / economics*, instrumentation, methods* Multiple Myeloma / immunology, pathology* Plasma Cells / immunology, pathology* Predictive Value of Tests Prospective Studies |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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