Document Detail


Single-stranded DNA as a cleavable linker for bioorthogonal click chemistry-based proteomics.
MedLine Citation:
PMID:  23627610     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this communication, we report a new class of cleavable linker based on automatically synthesized, single-stranded DNAs. We incorporated a DNA oligo into an azide-functionalized biotin (biotin-DNA-N3) and used the probe to enrich for alkyne-tagged glycoproteins from mammalian cell lysates. Highly efficient and selective release of the captured proteins from streptavidin agarose resins was achieved using DNase treatment under very mild conditions. A total of 36 sialylated glycoproteins were identified from the lysates of HL60 cells, an acute human promyeloid leukemia cell line. These sialylated glycoproteins were involved in many different biological processes ranging from glycan biosynthesis to cell adhesion events.
Authors:
Tianqing Zheng; Hao Jiang; Peng Wu
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-05-17
Journal Detail:
Title:  Bioconjugate chemistry     Volume:  24     ISSN:  1520-4812     ISO Abbreviation:  Bioconjug. Chem.     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-06-19     Completed Date:  2014-01-20     Revised Date:  2014-07-03    
Medline Journal Info:
Nlm Unique ID:  9010319     Medline TA:  Bioconjug Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  859-64     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Alkynes / chemistry
Azides / chemistry
Biotin / chemistry
Click Chemistry*
DNA, Single-Stranded / chemical synthesis,  chemistry*
Glycoproteins / chemistry*
HL-60 Cells
Humans
Molecular Structure
Proteomics*
Grant Support
ID/Acronym/Agency:
P30 CA013330/CA/NCI NIH HHS; R01 GM093282/GM/NIGMS NIH HHS; R01GM093282/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Alkynes; 0/Azides; 0/DNA, Single-Stranded; 0/Glycoproteins; 6SO6U10H04/Biotin
Comments/Corrections

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