Document Detail


Single-stranded DNA aptamers that bind differentiated but not parental cells: subtractive systematic evolution of ligands by exponential enrichment.
MedLine Citation:
PMID:  12668310     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this paper, single-stranded (ss)DNA aptamers with capability to distinguish differentiated PC12 cells from normal PC12 cells were selected by subtractive systematic evolution of ligands by exponential enrichment (SELEX) method. Before each round of selection, randomized ssDNAs were incubated with regular PC12 cells to eliminate those that recognize the common cellular components of both differentiated and undifferentiated PC12 cells. After six rounds of cell-based selection, both of individual aptamers and aptamers of the sixth round pool were found binding to differentiated PC12 cells, but not to the parental PC12 cells. The aptamers of the starting pool showed no such binding. Sequence analysis illustrated that the amount of G content in central random region of these aptamers was much higher than that of the starting pool, which would be expected to be average. The aptamers obtained from this method were also able to identify differentiated PC12 cells from a mixture of both normal and differentiated cells. The results indicate that subtractive SELEX is a useful tool in finding ligands to specific biological markers that distinguish a subtype of cells from cells of homologous origin, such as carcinoma cells among normal epithelial tissues. Both these aptamers and their markers may play important roles in basic research and clinical diagnosis.
Authors:
Chenglong Wang; Ming Zhang; Guang Yang; Dajing Zhang; Hongmei Ding; Huixing Wang; Ming Fan; Beifen Shen; Ningsheng Shao
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; Validation Studies    
Journal Detail:
Title:  Journal of biotechnology     Volume:  102     ISSN:  0168-1656     ISO Abbreviation:  J. Biotechnol.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-04-01     Completed Date:  2004-02-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  15-22     Citation Subset:  IM    
Affiliation:
Beijing Institute of Basic Medical Sciences, PO Box 130(3), Beijing 100850, People's Republic of China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Binding Sites / genetics
Cell Differentiation / genetics*
Combinatorial Chemistry Techniques / methods*
DNA, Single-Stranded / genetics*,  metabolism*
Evolution, Molecular*
Ligands
Macromolecular Substances
PC12 Cells / classification*,  cytology,  metabolism*
Rats
Chemical
Reg. No./Substance:
0/DNA, Single-Stranded; 0/Ligands; 0/Macromolecular Substances

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Progress towards single-molecule DNA sequencing: a one color demonstration.
Next Document:  Effects of temperature and pressure on Rhizomucor miehei lipase stability.