| Single-strand conformation polymorphism analysis for the study of adenoviral diversity in urban rivers. | |
| | |
MedLine Citation:
|
PMID: 20117139 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The diversity of human adenoviruses (AdVs) in river waters was studied by single-strand conformational polymorphism (SSCP) analysis. Water samples were collected between 2002 and 2003 from 4 rivers in the Gyeonggi Province, South Korea. Forty-six (79.3%) of the 58 samples were positive for AdVs as determined on PCR amplification. Nine different SSCP profiles (profiles A to I) were detected in all the AdVs-positive samples by SSCP analysis, and most of the AdVs-positive samples (38 of 46 samples; 82.6%) showed the SSCP profile D. Nine different sequences were obtained in the SSCP profiles; sequence alignments and phylogenetic analysis identified 5 different sequences that were closely related to the human AdV type 41, and the 4 different sequences that were closely related to human AdV types 3, 4, 12, and 40. Two AdVs genomic variants were detected (types 3 and 41 in A, types 12 and 41 in B, and 2 genomic variants of type 41 in C) in SSCP profiles A, B, and C, respectively. SSCP analysis could be a useful technique for the identification of genetic variants of AdVs and for studying AdVs diversity in urban rivers. |
| | |
Authors:
|
Cheonghoon Lee; Sang-Jong Kim |
Related Documents
:
|
16730419 - Association of tnfsf11 gene promoter polymorphisms with bone mineral density in postmen... 16199539 - An snp in protamine 1: a possible genetic cause of male infertility? 1409609 - Mapping genes in the mouse using single-strand conformation polymorphism analysis of re... 17498919 - Distribution analysis of nonsynonymous polymorphisms within the human kinase gene family. 16614319 - Common genetic variation in five thrombosis genes and relations to plasma hemostatic pr... 21899519 - B*13:50, a novel hla-b*13 allele, identified by sequence-based typing. 2170329 - Cloning and characterization of tfds, the repressor-activator gene of tfdb, from the 2,... 3539589 - Promoters of escherichia coli: a hierarchy of in vivo strength indicates alternate stru... 15198809 - Smart amplification combined with cdna size fractionation in order to obtain large full... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-01-29 |
Journal Detail:
|
Title: Journal of virological methods Volume: 165 ISSN: 1879-0984 ISO Abbreviation: J. Virol. Methods Publication Date: 2010 May |
Date Detail:
|
Created Date: 2010-04-07 Completed Date: 2010-07-22 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8005839 Medline TA: J Virol Methods Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 145-50 Citation Subset: IM |
Copyright Information:
|
Copyright 2010 Elsevier B.V. All rights reserved. |
Affiliation:
|
School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul, South Korea. |
| Data Bank Information | |
Bank Name/Acc. No.:
|
GENBANK/GQ477371; GQ477372; GQ477373; GQ477374; GQ477375; GQ477376; GQ477377; GQ477378; GQ477379 |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adenoviruses, Human
/
classification*,
genetics Base Sequence Environmental Monitoring / methods* Humans Molecular Sequence Data Phylogeny Polymerase Chain Reaction Polymorphism, Single-Stranded Conformational* Republic of Korea Rivers / virology* Water Microbiology* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A novel, rapid method to detect infectious HIV-1 from plasma from persons infected with HIV-1.
Next Document: Rapid automation of a cell-based assay using a modular approach: case study of a flow-based Varicell...