Document Detail


Single-step purification of two hyperglycaemic neurohormones from the sinus gland of Procambarus bouvieri. Comparative peptide mapping by means of high-performance liquid chromatography.
MedLine Citation:
PMID:  2902105     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A crude aqueous extract from 2000 sinus glands of the Mexican crayfish Procambarus bouvieri (Ortmann) was fractionated on a muBondapak-Phenyl column. Two isoforms of the crustacean hyperglycaemic hormone, designated CHH-B and CHH-C in order of elution, were isolated in pure form. Their biochemical characterization showed a remarkable degree of homology. A tryptic digest of each isohormone was fractionated on an Ultrasphere-ODS column. Only one tryptic peptide in CHH-C was eluted later than its homologous peptide in CHH-B. On acid hydrolysis both tryptic peptides had the same composition but, as they contain Asp and Glu, we suspect that the difference residues in a double reciprocal amidation-deamidation of two acidic residues.
Authors:
A Huberman; M B Aguilar
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography     Volume:  443     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1988 Jun 
Date Detail:
Created Date:  1988-10-31     Completed Date:  1988-10-31     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  337-42     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Animals
Astacoidea / metabolism*
Chromatography, High Pressure Liquid
Exocrine Glands / analysis*
Female
Hydrolysis
Indicators and Reagents
Male
Neurotransmitter Agents / isolation & purification*
Peptide Mapping
Proteins / analysis
Tissue Extracts / analysis
Trypsin
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Indicators and Reagents; 0/Neurotransmitter Agents; 0/Proteins; 0/Tissue Extracts; EC 3.4.21.4/Trypsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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