Document Detail


Single step purification of locust adipokinetic hormones I and II by reversed-phase high-performance liquid chromatography, and amino-acid composition of the hormone II.
MedLine Citation:
PMID:  6724531     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Locust adipokinetic hormones I (AKH I) and II (AKH II) are separated by reversed-phase high-performance liquid chromatography using a mu-Bondapak phenyl column with a trifluoroacetic acid/acetonitrile gradient. The eluant was monitored at 210 nm and the hyperlipaemic activity was detected using a bioassay. The amino-acid composition of AKH II was determined after acid hydrolysis with HCl or methane-sulfonic acid. It contained the following amino-acid residues in almost equimolar amounts: Asp, Ser, Glu, Gly, Ala, Leu, Phe and Trp.
Authors:
G Gäde; G J Goldsworthy; G Kegel; R Keller
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Hoppe-Seyler's Zeitschrift für physiologische Chemie     Volume:  365     ISSN:  0018-4888     ISO Abbreviation:  Hoppe-Seyler's Z. Physiol. Chem.     Publication Date:  1984 Mar 
Date Detail:
Created Date:  1984-07-02     Completed Date:  1984-07-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985060R     Medline TA:  Hoppe Seylers Z Physiol Chem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  393-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Animals
Chromatography, High Pressure Liquid
Grasshoppers / analysis*
Hemolymph / analysis
Insect Hormones / isolation & purification*
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Insect Hormones; 53027-55-7/adipokinetic hormone (locust)

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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