| Single step purification of locust adipokinetic hormones I and II by reversed-phase high-performance liquid chromatography, and amino-acid composition of the hormone II. | |
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MedLine Citation:
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PMID: 6724531 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Locust adipokinetic hormones I (AKH I) and II (AKH II) are separated by reversed-phase high-performance liquid chromatography using a mu-Bondapak phenyl column with a trifluoroacetic acid/acetonitrile gradient. The eluant was monitored at 210 nm and the hyperlipaemic activity was detected using a bioassay. The amino-acid composition of AKH II was determined after acid hydrolysis with HCl or methane-sulfonic acid. It contained the following amino-acid residues in almost equimolar amounts: Asp, Ser, Glu, Gly, Ala, Leu, Phe and Trp. |
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Authors:
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G Gäde; G J Goldsworthy; G Kegel; R Keller |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Hoppe-Seyler's Zeitschrift für physiologische Chemie Volume: 365 ISSN: 0018-4888 ISO Abbreviation: Hoppe-Seyler's Z. Physiol. Chem. Publication Date: 1984 Mar |
Date Detail:
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Created Date: 1984-07-02 Completed Date: 1984-07-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985060R Medline TA: Hoppe Seylers Z Physiol Chem Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 393-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis Animals Chromatography, High Pressure Liquid Grasshoppers / analysis* Hemolymph / analysis Insect Hormones / isolation & purification* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Insect Hormones; 53027-55-7/adipokinetic hormone (locust) |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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