Document Detail


Single step isolation of immunofluorescence-stained, viable lymphocytes by fluorescence-activated cell sorter using ethidium bromide.
MedLine Citation:
PMID:  3092814     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The paper presents a method for preparation of viable, immunofluorescence-stained lymphocytes in a single isolation step by fluorescence-activated cell sorter. Discrimination between viable, immunofluorescence positive cells and nonspecifically stained dead cells was carried out on the basis of the DNA-specific dye ethidium bromide. Ethidium bromide enters cells with damaged plasma membrane only. The method is described by the example of isolation of Lyt-2 positive mouse lymph node cells.
Authors:
L Karawajew; B Schlott
Related Documents :
6831524 - Cell type-specific binding of ricinus lectin to murine cerebellar cell surfaces in vitro.
12801424 - Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-...
19485404 - High-density microcavity array for cell detection: single-cell analysis of hematopoieti...
2817394 - Testing for electrotransfection parameters by use of the fluorescent dye lucifer yellow...
8288864 - Cell phenotypes and differentiative transitions in mouse submandibular salivary gland d...
15170604 - Single-cell analysis of yeast, mammalian cells, and fungal spores with a microfluidic p...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biomedica biochimica acta     Volume:  45     ISSN:  0232-766X     ISO Abbreviation:  Biomed. Biochim. Acta     Publication Date:  1986  
Date Detail:
Created Date:  1986-10-10     Completed Date:  1986-10-10     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8304435     Medline TA:  Biomed Biochim Acta     Country:  GERMANY, EAST    
Other Details:
Languages:  eng     Pagination:  K1-3     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Ethidium
Flow Cytometry / methods
Fluorescein-5-isothiocyanate
Fluoresceins
Fluorescent Antibody Technique
Fluorescent Dyes
Lymph Nodes / cytology
Lymphocytes / cytology*
Male
Mice
Mice, Inbred Strains
Thiocyanates
Chemical
Reg. No./Substance:
0/Fluoresceins; 0/Fluorescent Dyes; 0/Thiocyanates; 3326-32-7/Fluorescein-5-isothiocyanate; 3546-21-2/Ethidium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Chemical characterization of cell-CAM 105, a cell-adhesion molecule isolated from rat liver membrane...
Next Document:  Effects of bacterial sepsis on protein metabolism in infants during the first week.