Document Detail

Single step isolation of immunofluorescence-stained, viable lymphocytes by fluorescence-activated cell sorter using ethidium bromide.
MedLine Citation:
PMID:  3092814     Owner:  NLM     Status:  MEDLINE    
The paper presents a method for preparation of viable, immunofluorescence-stained lymphocytes in a single isolation step by fluorescence-activated cell sorter. Discrimination between viable, immunofluorescence positive cells and nonspecifically stained dead cells was carried out on the basis of the DNA-specific dye ethidium bromide. Ethidium bromide enters cells with damaged plasma membrane only. The method is described by the example of isolation of Lyt-2 positive mouse lymph node cells.
L Karawajew; B Schlott
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biomedica biochimica acta     Volume:  45     ISSN:  0232-766X     ISO Abbreviation:  Biomed. Biochim. Acta     Publication Date:  1986  
Date Detail:
Created Date:  1986-10-10     Completed Date:  1986-10-10     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8304435     Medline TA:  Biomed Biochim Acta     Country:  GERMANY, EAST    
Other Details:
Languages:  eng     Pagination:  K1-3     Citation Subset:  IM    
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MeSH Terms
Flow Cytometry / methods
Fluorescent Antibody Technique
Fluorescent Dyes
Lymph Nodes / cytology
Lymphocytes / cytology*
Mice, Inbred Strains
Reg. No./Substance:
0/Fluoresceins; 0/Fluorescent Dyes; 0/Thiocyanates; 3326-32-7/Fluorescein-5-isothiocyanate; 3546-21-2/Ethidium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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