| Single-prolonged stress induces apoptosis in the amygdala in a rat model of post-traumatic stress disorder. | |
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MedLine Citation:
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PMID: 19586638 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To detect the apoptosis-related Bax and Bcl-2 gene expression and apoptotic cell death in the amygdala region in the single-prolonged stress (SPS) rats. METHODS: A total of 100 male Wistar rats were randomly divided into a normal control group and SPS groups of 1d, 4d, 7d, and 14d. The expression of Bax and Bcl-2 was detected using immunohistochemistry and Western Blotting; TUNEL-staining and double-labeled flow cytometry (FCM) were employed for the detection and quantification of the apoptotic cells in the amygdala; morphological change of the subcellular structure in amygdala was observed by using the transmission electron microscopy (TEM). RESULTS: The ratio of Bax/Bcl-2 peaked at SPS 4d and then gradually decreased. The apoptosis peaked at SPS 4d. The TUNEL-positive cells were found in each SPS group and the TUNEL-positive cells rate peaked at SPS 4d. The morphological change of amygdala cells in each SPS group bears typical apoptotic characteristics. CONCLUSIONS: In the SPS rat brain, we found apoptotic process in the amygdala region which may relate to the pathogenesis of amygdala abnormal function in PTSD. |
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Authors:
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Jinlan Ding; Fang Han; Yuxiu Shi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-07-07 |
Journal Detail:
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Title: Journal of psychiatric research Volume: 44 ISSN: 1879-1379 ISO Abbreviation: J Psychiatr Res Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2010-01-28 Completed Date: 2010-03-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376331 Medline TA: J Psychiatr Res Country: England |
Other Details:
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Languages: eng Pagination: 48-55 Citation Subset: IM |
Affiliation:
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Department of Histology and Embryology, Basic Medical College, China Medical University, Shenyang, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amygdala
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pathology,
physiopathology*,
ultrastructure Animals Apoptosis / physiology* Disease Models, Animal Gene Expression Regulation / physiology* In Situ Nick-End Labeling Male Microscopy, Electron, Transmission / methods Proto-Oncogene Proteins c-bcl-2 / genetics, metabolism Rats Rats, Wistar Stress Disorders, Post-Traumatic / etiology*, pathology* Stress, Physiological* Time Factors bcl-2-Associated X Protein / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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