Document Detail

Single potential electrophoresis microchip with reduced bias using pressure pulse injection.
MedLine Citation:
PMID:  16639704     Owner:  NLM     Status:  MEDLINE    
We propose two variants of a new injection technique for use in electrophoresis microchips, called "front gate pressure injection" and "back gate pressure injection", that both enable a controlled and reproducible sample introduction with reduced bias compared to electrokinetic gated injection. A continuous flow of a test solution of fluorescein/rhodamine B in 20 mM Tris/boric acid buffer (pH 8.6) sample test solution is electrokinetically driven near to the entrance of the separation channel, using a single voltage (3 kV) that is constant in time. A sample plug is injected in the separation channel by a pressure pulse of the order of 0.1 s. The latter is generated using the mechanical deflection of a PDMS membrane that is loosely placed on a dedicated chip reservoir. The analysis of the peak area ratio of the separated compounds demonstrates a nearly constant sample composition when using pressure-based injection. A small remaining injection bias for the shortest membrane deflection times can be attributed to a dilution effect of the charged compound due to the presence of an electrical field transverse to the sample flow boundary in the channel junction.
Frédéric Lacharme; Martin A M Gijs
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Electrophoresis     Volume:  27     ISSN:  0173-0835     ISO Abbreviation:  Electrophoresis     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-08-23     Completed Date:  2006-10-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2924-32     Citation Subset:  IM    
Institute of Microelectronics and Microsystems, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
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MeSH Terms
Dimethylpolysiloxanes / chemistry
Electrophoresis, Capillary / methods*
Electrophoresis, Microchip / methods*
Flow Injection Analysis / methods*
Reg. No./Substance:

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