| Single potential electrophoresis microchip with reduced bias using pressure pulse injection. | |
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MedLine Citation:
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PMID: 16639704 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We propose two variants of a new injection technique for use in electrophoresis microchips, called "front gate pressure injection" and "back gate pressure injection", that both enable a controlled and reproducible sample introduction with reduced bias compared to electrokinetic gated injection. A continuous flow of a test solution of fluorescein/rhodamine B in 20 mM Tris/boric acid buffer (pH 8.6) sample test solution is electrokinetically driven near to the entrance of the separation channel, using a single voltage (3 kV) that is constant in time. A sample plug is injected in the separation channel by a pressure pulse of the order of 0.1 s. The latter is generated using the mechanical deflection of a PDMS membrane that is loosely placed on a dedicated chip reservoir. The analysis of the peak area ratio of the separated compounds demonstrates a nearly constant sample composition when using pressure-based injection. A small remaining injection bias for the shortest membrane deflection times can be attributed to a dilution effect of the charged compound due to the presence of an electrical field transverse to the sample flow boundary in the channel junction. |
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Authors:
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Frédéric Lacharme; Martin A M Gijs |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Electrophoresis Volume: 27 ISSN: 0173-0835 ISO Abbreviation: Electrophoresis Publication Date: 2006 Jul |
Date Detail:
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Created Date: 2006-08-23 Completed Date: 2006-10-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8204476 Medline TA: Electrophoresis Country: Germany |
Other Details:
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Languages: eng Pagination: 2924-32 Citation Subset: IM |
Affiliation:
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Institute of Microelectronics and Microsystems, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland. Frederic.Lacharme@epfl.ch |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Dimethylpolysiloxanes
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chemistry Electrophoresis, Capillary / methods* Electrophoresis, Microchip / methods* Flow Injection Analysis / methods* Pressure |
| Chemical | |
Reg. No./Substance:
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0/Dimethylpolysiloxanes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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