Document Detail


Single nucleotide polymorphisms of the heat shock protein 90 gene in varicocele-associated infertility.
MedLine Citation:
PMID:  15992426     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Varicoceles are associated with impaired testicular function and male infertility, but the molecular mechanisms by which fertility is affected have not been satisfactorily explained. Spermatogenesis might be affected by increased scrotal temperature, such as that caused by varicocele. HSP90 is a molecular chaperone expressed in germ cells and is related to spermatogenesis, motility, and both heat and oxidative stress. Possible correlations between coding single region nucleotide polymorphisms (cSNPs) in the HSP90 gene in patients with varicocele associated with infertility were analyzed, and polymorphisms in these exons were characterized through DNA sequencing. MATERIALS AND METHODS: PCR-SSCP and DNA sequencing were used to search for mutations in 18 infertile patients with varicocele, 11 patients with idiopathic infertility and 12 fertile men. DNA was extracted from leucocytes for PCR amplification and SSCP analysis. DNA from samples with an altered band pattern in the SSCP was then sequenced to search for polymorphisms. RESULTS: Three silent polymorphisms that do not lead to amino acid substitutions were identified. CONCLUSION: Mutations in the HSP90 gene do not appear to be a common cause of male factor infertility. The low incidence of gene variation, or SNPs, in infertile men demonstrates that this gene is highly conserved and thus confirms its key role in spermatogenesis and response to heat stress.
Authors:
Pericles A Hassun Filho; Agnaldo P Cedenho; Samira B Lima; Valdemar Ortiz; Miguel Srougi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International braz j urol : official journal of the Brazilian Society of Urology     Volume:  31     ISSN:  1677-5538     ISO Abbreviation:  Int Braz J Urol     Publication Date:    2005 May-Jun
Date Detail:
Created Date:  2005-07-04     Completed Date:  2005-10-24     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  101158091     Medline TA:  Int Braz J Urol     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  236-42     Citation Subset:  IM    
Affiliation:
Department of Surgery, Division of Urology, Federal University of Sao Paulo, Sao Paulo, Brazil. pericles.hassun@gmail.com
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MeSH Terms
Descriptor/Qualifier:
HSP90 Heat-Shock Proteins / genetics*
Humans
Infertility, Male / etiology*,  genetics
Male
Mutation / genetics*
Polymerase Chain Reaction
Polymorphism, Single Nucleotide / genetics*
Polymorphism, Single-Stranded Conformational
Prospective Studies
Sequence Analysis, DNA
Severity of Illness Index
Varicocele / complications*,  genetics
Chemical
Reg. No./Substance:
0/HSP90 Heat-Shock Proteins
Comments/Corrections
Comment In:
Int Braz J Urol. 2005 May-Jun;31(3):243-4   [PMID:  16052745 ]

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