Document Detail

Single nucleotide polymorphisms of the heat shock protein 90 gene in varicocele-associated infertility.
MedLine Citation:
PMID:  15992426     Owner:  NLM     Status:  MEDLINE    
PURPOSE: Varicoceles are associated with impaired testicular function and male infertility, but the molecular mechanisms by which fertility is affected have not been satisfactorily explained. Spermatogenesis might be affected by increased scrotal temperature, such as that caused by varicocele. HSP90 is a molecular chaperone expressed in germ cells and is related to spermatogenesis, motility, and both heat and oxidative stress. Possible correlations between coding single region nucleotide polymorphisms (cSNPs) in the HSP90 gene in patients with varicocele associated with infertility were analyzed, and polymorphisms in these exons were characterized through DNA sequencing. MATERIALS AND METHODS: PCR-SSCP and DNA sequencing were used to search for mutations in 18 infertile patients with varicocele, 11 patients with idiopathic infertility and 12 fertile men. DNA was extracted from leucocytes for PCR amplification and SSCP analysis. DNA from samples with an altered band pattern in the SSCP was then sequenced to search for polymorphisms. RESULTS: Three silent polymorphisms that do not lead to amino acid substitutions were identified. CONCLUSION: Mutations in the HSP90 gene do not appear to be a common cause of male factor infertility. The low incidence of gene variation, or SNPs, in infertile men demonstrates that this gene is highly conserved and thus confirms its key role in spermatogenesis and response to heat stress.
Pericles A Hassun Filho; Agnaldo P Cedenho; Samira B Lima; Valdemar Ortiz; Miguel Srougi
Related Documents :
9145546 - Success in mating: a coordinated approach to fitness through genotypes incorporating ge...
19143906 - The genetics and epidemiology of female sexual dysfunction: a review.
25253456 - Turnover and accumulation of genetic diversity across large time-scale cycles of isolat...
20300286 - A386g transition in dazl gene is not associated with spermatogenic failure in tamil nad...
22127186 - Genetic variation of mitochondrial dna in chinese populations of pectinophora gossypiel...
8185826 - Mutations in a shuttle vector exposed to activated mitomycin c.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International braz j urol : official journal of the Brazilian Society of Urology     Volume:  31     ISSN:  1677-5538     ISO Abbreviation:  Int Braz J Urol     Publication Date:    2005 May-Jun
Date Detail:
Created Date:  2005-07-04     Completed Date:  2005-10-24     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  101158091     Medline TA:  Int Braz J Urol     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  236-42     Citation Subset:  IM    
Department of Surgery, Division of Urology, Federal University of Sao Paulo, Sao Paulo, Brazil.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
HSP90 Heat-Shock Proteins / genetics*
Infertility, Male / etiology*,  genetics
Mutation / genetics*
Polymerase Chain Reaction
Polymorphism, Single Nucleotide / genetics*
Polymorphism, Single-Stranded Conformational
Prospective Studies
Sequence Analysis, DNA
Severity of Illness Index
Varicocele / complications*,  genetics
Reg. No./Substance:
0/HSP90 Heat-Shock Proteins
Comment In:
Int Braz J Urol. 2005 May-Jun;31(3):243-4   [PMID:  16052745 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Preliminary experience with extraperitoneal endoscopic radical prostatectomy through duplication of ...
Next Document:  Deep-seated sarcomas of the penis.