Document Detail

Single-molecule studies of DNA and RNA four-way junctions.
MedLine Citation:
PMID:  14748709     Owner:  NLM     Status:  MEDLINE    
Branched helical junctions are common in nucleic acids. In DNA, the four-way junction (Holliday junction) is an essential intermediate in homologous recombination and is a highly dynamic structure, capable of stacking conformer transitions and branch migration. Our single-molecule fluorescence studies provide unique insight into the energy landscape of Holliday junctions by visualizing these processes directly. In the hairpin ribozyme, an RNA four-way junction is an important structural element that enhances active-site formation by several orders of magnitude. Our single-molecule studies suggest a plausible mechanism for how the junction achieves this remarkable feat; the structural dynamics of the four-way junction bring about frequent contacts between the loops that are needed to form the active site. The most definitive evidence for this is the observation of three-state folding in single-hairpin ribozymes, the intermediate state of which is populated due to the intrinsic properties of the junction.
S A McKinney; E Tan; T J Wilson; M K Nahas; A-C Déclais; R M Clegg; D M J Lilley; T Ha
Related Documents :
22236779 - Electrochemical growth of gold nanoparticles on horizontally aligned carbon nanotubes: ...
19759859 - Dna loci cross-talk through thermodynamics.
11909119 - Single molecule statistics and the polynucleotide unzipping transition.
22097999 - Diagnosing leprosy: revisiting the role of the slit-skin smear with critical analysis o...
8952489 - Sequence-dependent effects of spermine on the thermodynamics of the b-dna to z-dna tran...
21348849 - Recombination proteins and telomere stability in plants.
1423869 - 32p-postlabeling analysis of the formation and persistence of dna adducts in mammary gl...
17186119 - The wiscdslox t-dna collection: an arabidopsis community resource generated by using an...
9949439 - Strategies for preimplantation genetic diagnosis of single gene disorders by dna amplif...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical Society transactions     Volume:  32     ISSN:  0300-5127     ISO Abbreviation:  Biochem. Soc. Trans.     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-01-29     Completed Date:  2004-09-24     Revised Date:  2014-10-28    
Medline Journal Info:
Nlm Unique ID:  7506897     Medline TA:  Biochem Soc Trans     Country:  England    
Other Details:
Languages:  eng     Pagination:  41-5     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
DNA / chemistry*,  metabolism*
DNA, Cruciform / chemistry,  metabolism
Fluorescence Resonance Energy Transfer
Magnesium / pharmacology
Nucleic Acid Conformation
RNA / chemistry*,  genetics,  metabolism*
RNA, Catalytic / chemistry,  genetics,  metabolism
Grant Support
11722//Cancer Research UK; R01 GM065367/GM/NIGMS NIH HHS
Reg. No./Substance:
0/DNA, Cruciform; 0/RNA, Catalytic; 63231-63-0/RNA; 9007-49-2/DNA; I38ZP9992A/Magnesium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Improved RNA cleavage by LNAzyme derivatives of DNAzymes.
Next Document:  In vitro and in vivo effects of oxidative damage to deoxyguanosine.