Document Detail


Single-molecule studies of DNA and RNA four-way junctions.
MedLine Citation:
PMID:  14748709     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Branched helical junctions are common in nucleic acids. In DNA, the four-way junction (Holliday junction) is an essential intermediate in homologous recombination and is a highly dynamic structure, capable of stacking conformer transitions and branch migration. Our single-molecule fluorescence studies provide unique insight into the energy landscape of Holliday junctions by visualizing these processes directly. In the hairpin ribozyme, an RNA four-way junction is an important structural element that enhances active-site formation by several orders of magnitude. Our single-molecule studies suggest a plausible mechanism for how the junction achieves this remarkable feat; the structural dynamics of the four-way junction bring about frequent contacts between the loops that are needed to form the active site. The most definitive evidence for this is the observation of three-state folding in single-hairpin ribozymes, the intermediate state of which is populated due to the intrinsic properties of the junction.
Authors:
S A McKinney; E Tan; T J Wilson; M K Nahas; A-C Déclais; R M Clegg; D M J Lilley; T Ha
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical Society transactions     Volume:  32     ISSN:  0300-5127     ISO Abbreviation:  Biochem. Soc. Trans.     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-01-29     Completed Date:  2004-09-24     Revised Date:  2014-10-28    
Medline Journal Info:
Nlm Unique ID:  7506897     Medline TA:  Biochem Soc Trans     Country:  England    
Other Details:
Languages:  eng     Pagination:  41-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
DNA / chemistry*,  metabolism*
DNA, Cruciform / chemistry,  metabolism
Fluorescence Resonance Energy Transfer
Magnesium / pharmacology
Nucleic Acid Conformation
RNA / chemistry*,  genetics,  metabolism*
RNA, Catalytic / chemistry,  genetics,  metabolism
Grant Support
ID/Acronym/Agency:
11722//Cancer Research UK; R01 GM065367/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Cruciform; 0/RNA, Catalytic; 63231-63-0/RNA; 9007-49-2/DNA; I38ZP9992A/Magnesium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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