| Single living cell detection of telomerase over-expression for cancer detection by an optical fiber nanobiosensor. | |
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MedLine Citation:
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PMID: 19963365 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An optical fiber nanobiosensor was constructed to successfully detect a general cancer biomarker, telomerase at single cell level with its nanoscale tip. The nanotip immobilized with a specific antibody was inserted into a MCF-7 breast cancer cell nucleus to capture telomerases directly, after which an in vitro enzymatic sandwich immunoassay was performed to achieve sensitive single living cell detection. The nanotip inserted into MCF-7 cell nucleus provides significantly higher average (F-F(0))/F(0) ratio than that of human mesenchymal stem cell (hMSC) nucleus, demonstrating the successful detection of the telomerase over-expression in cancer cells as compared to normal cells. The detection in the cytoplasm shows much smaller average ratio than that in the nucleus of MCF-7 cells while clearly verifies the nuclear localization of telomerase. The successful detection of telomerase over-expression in a single living cell for the first time may provide a potential method for cancer detection, and also demonstrate a universal approach that can be used to detect other low expression proteins in a single living cell. |
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Authors:
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Xin Ting Zheng; Chang Ming Li |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2009-11-11 |
Journal Detail:
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Title: Biosensors & bioelectronics Volume: 25 ISSN: 1873-4235 ISO Abbreviation: Biosens Bioelectron Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-03 Completed Date: 2010-04-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9001289 Medline TA: Biosens Bioelectron Country: England |
Other Details:
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Languages: eng Pagination: 1548-52 Citation Subset: IM |
Copyright Information:
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Copyright 2009 Elsevier B.V. All rights reserved. |
Affiliation:
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School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, Singapore. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biosensing Techniques
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instrumentation* Breast Neoplasms / diagnosis*, metabolism Cell Line, Tumor Cell Separation / instrumentation Equipment Design Equipment Failure Analysis Fiber Optic Technology / instrumentation* Gene Expression Profiling / instrumentation Humans Nanotechnology / instrumentation* Reproducibility of Results Sensitivity and Specificity Spectrometry, Fluorescence / instrumentation* Telomerase / analysis* Tumor Markers, Biological / analysis* Up-Regulation |
| Chemical | |
Reg. No./Substance:
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0/Tumor Markers, Biological; EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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