| Single-laboratory validation of a refined AOAC HPLC method 2005.06 for oysters, cockles, and clams in U.K. shellfish. | |
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MedLine Citation:
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PMID: 21140661 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In 2009, a refined HPLC method based on AOAC Official Method 2005.06 was developed and validated for the determination of paralytic shellfish poisoning (PSP) in mussels. A single-laboratory validation study of this method was undertaken here for the analysis of PSP toxins in oysters, cockles, clams, and razor clams. The method was characterized for selectivity, sensitivity, linearity, precision, repeatability, recovery, ruggedness, and uncertainty of measurement. Validation data were utilized to determine method performance characteristics for non-mussel bivalves for all commercially available certified reference toxins, extending the method to dcNEO and dcGTX2,3, where available. A period of parallel testing of oysters, cockles, and clams enabled a comparison of sample toxicities obtained using mouse bioassay (MBA) and HPLC methodologies, although only a very low number of PSP-positive samples were obtained through the United Kingdom official control monitoring program. Results from the MBA and HPLC methods were well-correlated for PSP-negative samples, but the low number of naturally contaminated PSP-positive samples has prevented any comparative statistical assessment of method performance for non-mussels between the two official methods. However, some evidence for potentially significant differences in total saxitoxin equivalents obtained by the two methods in some species has highlighted the need for further comparative testing in non-mussel samples to be conducted prior to implementation of the HPLC method in routine official control monitoring programs. |
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Authors:
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Andrew D Turner; Robert G Hatfield; Monika Rapkova-Dhanji; Deirdre M Norton; Myriam Algoet; David N Lees |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of AOAC International Volume: 93 ISSN: 1060-3271 ISO Abbreviation: J AOAC Int Publication Date: 2010 Sep-Oct |
Date Detail:
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Created Date: 2010-12-09 Completed Date: 2010-12-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9215446 Medline TA: J AOAC Int Country: United States |
Other Details:
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Languages: eng Pagination: 1482-93 Citation Subset: IM |
Affiliation:
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Centre for Environment, Fisheries and Aquaculture Science, Barrack Rd, The Nothe, Weymouth, Dorset, DT4 8UB, United Kingdom. andrew.turner@cefas.co.uk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bivalvia / chemistry* Cardiidae / chemistry* Chromatography, High Pressure Liquid / methods* Limit of Detection Marine Toxins / analysis* Ostreidae / chemistry* Reproducibility of Results Shellfish / toxicity* Shellfish Poisoning / etiology* |
| Chemical | |
Reg. No./Substance:
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0/Marine Toxins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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