| Single intravenous injection of plasmid DNA encoding human paraoxonase-1 inhibits hyperlipidemia in rats. | |
| | |
MedLine Citation:
|
PMID: 20580687 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Paraoxonase-1 (PON1, EC 3.1.8.1) is a high-density lipoprotein (HDL)-associated antioxidant enzyme, and its activity correlates negatively with the level of plasma low-density lipoprotein cholesterol (LDL-C) and triglyceridemia (TG). In this study, we examined the therapeutic effect of plasmid DNA containing the human PON1 gene (pcDNA/PON1) in hyperlipidemic model rats. The rats were fed a high-fat and high-cholesterol diet for 25 days to produce a hyperlipidemic animal model. Single intravenous injection of pcDNA/PON1 into model rats prevented dyslipidemia and hepatic lipid accumulation. The mechanisms of pcDNA/PON1 in treating hyperlipidemia were associated with increases of serum antioxidant PON1 and SOD activities, and with reduction of the levels of total cholesterol (TC), LDL-C and TG. The results suggest the potential therapeutic effect of pcDNA/PON1 on hyperlipidemia. |
| | |
Authors:
|
Ai Ling Fu; Shao Ping Wu |
Related Documents
:
|
11372707 - Metabolic studies on lean and fat pekin ducks selected for breast muscle thickness meas... 22243657 - Reducing sodium levels in frankfurters using naturally brewed soy sauce. 9323587 - Clearance of postprandial and lipolytically modified human hdl in rabbits and rats. 16829397 - Interrelations between lipoprotein and the elimination of exogenous triglycerides from ... 15649137 - The conflicting role of brain cholesterol in alzheimer's disease: lessons from the brai... 1121487 - Effect of cholestyramine on tissue pools of cholesterol a preliminary report. |
Publication Detail:
|
Type: Journal Article Date: 2010-05-24 |
Journal Detail:
|
Title: Biochemical and biophysical research communications Volume: 397 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2010 Jun |
Date Detail:
|
Created Date: 2010-07-08 Completed Date: 2010-08-10 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
|
Languages: eng Pagination: 257-62 Citation Subset: IM |
Copyright Information:
|
Copyright (c) 2010 Elsevier Inc. All rights reserved. |
Affiliation:
|
School of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China. A.L.Fu@hotmail.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Aryldialkylphosphatase / blood, genetics* Cholesterol, LDL / blood DNA / administration & dosage* Gene Therapy / methods* Humans Hyperlipidemias / therapy* Injections, Intravenous Liver / metabolism Male Plasmids / administration & dosage Rats Rats, Sprague-Dawley Superoxide Dismutase / blood Triglycerides / blood |
| Chemical | |
Reg. No./Substance:
|
0/Cholesterol, LDL; 0/Triglycerides; 9007-49-2/DNA; EC 1.15.1.1/Superoxide Dismutase; EC 3.1.8.1/Aryldialkylphosphatase; EC 3.1.8.1/PON1 protein, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Urokinase receptor (uPAR) regulates complement receptor 3 (CR3)-mediated neutrophil phagocytosis.
Next Document: Mechanical stress-evoked but angiotensin II-independent activation of angiotensin II type 1 receptor...