| Single-input divergent flow IEF for preparative analysis of proteins. | |
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MedLine Citation:
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PMID: 18985663 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The instrument for continuous divergent flow IEF based on our principles set and outlined previously was further extended and tested. The separation and focusing area of a trapezoidal shape had a porous bed made from a nonwoven textile material with thickness decreasing from narrow input to wide output. A narrow end was used as a single input to continuously bring a single solution into separation space with a flow rate of 0.18 mL/min. Two pairs of electrodes were positioned close to both narrow and wide ends of the separation area with equilibrium state voltage of 75 V at the narrow input and 384 V at the wide output. Under dynamic equilibrium state, the zones of both pH gradient components and analytes were separated close to the input point and focused with increasing resolution while transporting through the separation space. The long-term stability experiments had shown the suitability of the device for preparative analysis; the zones of pI markers, hemoglobin and cytochrome C remained focused and separated over 15 h with deviations from the mean focusing positions ranging from 1.26 to 3.96% of the bed output width. |
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Authors:
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Miroslava Stastna; Karel Slais |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Electrophoresis Volume: 29 ISSN: 1522-2683 ISO Abbreviation: Electrophoresis Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-12-02 Completed Date: 2009-04-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8204476 Medline TA: Electrophoresis Country: Germany |
Other Details:
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Languages: eng Pagination: 4503-7 Citation Subset: IM |
Affiliation:
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Institute of Analytical Chemistry of the ASCR, Brno, Czech Republic. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cytochromes c
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isolation & purification Hemoglobins / isolation & purification Hydrogen-Ion Concentration Isoelectric Focusing / methods* Polyesters / chemistry Proteins / isolation & purification* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Hemoglobins; 0/Polyesters; 0/Proteins; 9007-43-6/Cytochromes c |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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