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Single-fraction γ-60Co radiation induces apoptosis in cultured rat C6 cells.
MedLine Citation:
PMID:  22588438     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
BACKGROUND AND OBJECTIVES: Radiotherapy is frequently applied in the treatment of malignant gliomas, but it is unclear if radiotherapy exerts its effects via induction of apoptosis. The present study was designed to determine whether a single-fraction γ-60Co radiation can induce apoptosis.
DESIGN AND SETTING: In vitro cytological controlled study performed at a military medical university from October 2006 to June 2008.
METHODS: C6 cells were treated with a single fraction of ?-60Co radiation at various doses (0, 4, 16, and 64 Gy). The 3-(4,5)-dimethylthiazol-2)-2,5-diphenyl tetrazolium bromide (MTT) assay, apoptosis assays using Annexin V-fluorescein isothiocyanate /propidium iodide or Hoechst 33258 staining, and the cell cycle assay were performed, and the expression of p53 and p21 proteins was evaluated.
RESULTS: The C6 cell numbers in the 16 Gy and 64 Gy groups were much lower than in the control group at 48, 96, and 144 hours after irradiation. The irradiated cells underwent apoptosis in a dose-dependent manner. Irradiation also impacted cell cycle progression, arresting cells in the G1 phase. The p53 protein expression was shown in both the nucleus and the cytoplasm of irradiated cells, whereas p53 was only expressed in the nucleus of control (untreated) cells. The p21 protein was expressed in irradiated cells but not in control cells.
CONCLUSIONS: Single-fraction ?-60Co radiation inhibited C6 cell growth by inducing apoptosis and G1 arrest, which correlated with the up-regulation of the p53-p21 pathway. The extent of apoptosis and G1 arrest was positively correlated with the dose of radiation. Better understanding of apoptosis induced by radiation therapy will help design optimal dosing schedules for radiation therapy, especially in combination with chemotherapy.
Authors:
Jiefang Bian; Xiling Wang; Jun Yun; Ruifeng Cao; Yunxin Cao; Jingwen Liang; B Fucheng Ma
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Annals of Saudi medicine     Volume:  32     ISSN:  0975-4466     ISO Abbreviation:  Ann Saudi Med     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8507355     Medline TA:  Ann Saudi Med     Country:  India    
Other Details:
Languages:  eng     Pagination:  269-75     Citation Subset:  IM    
Affiliation:
Jiefang Bian, Department of Vascular Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China T: (86)29-84773108, (86)13909231158, F: (86)29-84773108 jfbian@yahoo.com.
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