Document Detail


SINGLE-DOSE ASSAY TECHNIQUE FOR VARIOLA VIRUS.
MedLine Citation:
PMID:  14106945     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A biological assay for variola virus was needed that would (i) require a minimum of time and (ii) have acceptable precision. Preliminary titrations made in 11-day-old embryonated eggs and in suckling mice (6 to 24 hr of age) demonstrated a linear relationship between the concentration of variola virus injected and the mean reciprocal time to death (MTD) of both hosts. This linear response indicated that the injection of a single dose of virus suspension and the measurement of the MTD should result in an acceptable assay method. Seven replicate samples of liquid preparations (20% chorioallantoic membrane in Heart Infusion Broth) and the freeze-dried material obtained with these suspensions were assayed in triplicate for pock infectious units and for MTD. The variance of the pock counts was far greater than was expected from a Poisson distribution, and coefficients of variation ranged from 25 to 61%. Variances obtained with the single-dilution assay were all far below that expected from a Poisson distribution, and coefficients of variations ranged from 5.2 to 13.6%. The use of the MTD assay resulted in a saving of time, a saving in the number of hosts necessary per assay, and increased precision.
Authors:
J M RILEY; M D ORLANDO
Related Documents :
9889225 - Detection of rubella virus-specific immunoglobulin g in saliva by an amplification-base...
12738645 - Development of an enzyme-linked immunosorbent assay for immunoglobulin m antibodies aga...
6885955 - Extension of the elisa method to the measurement of the specific radioactivity of virus...
965475 - Solid-phase radioimmunoassay of rubella virus immunoglobulin g and immunoglobulin m ant...
1645595 - Differentiation between transmissible gastroenteritis virus and porcine respiratory cor...
22709385 - Human infection from avian-like influenza a (h1n1) viruses in pigs, china.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied microbiology     Volume:  12     ISSN:  0003-6919     ISO Abbreviation:  Appl Microbiol     Publication Date:  1964 Jan 
Date Detail:
Created Date:  1964-06-01     Completed Date:  1996-12-01     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605802     Medline TA:  Appl Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7-9     Citation Subset:  OM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Biological Assay*
Chick Embryo*
Mice*
Penicillins*
Research*
Streptomycin*
Variola virus*
Chemical
Reg. No./Substance:
0/Penicillins; 57-92-1/Streptomycin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  STAPHYLOCOCCI IN COMPETITION. IV. EFFECT OF STARCH AND KIND AND CONCENTRATION OF SUGAR ON STAPHYLOCO...
Next Document:  STAPHYLOCOCCI IN COMPETITION. V. EFFECT OF EGGS, EGGS PLUS CARBOHYDRATES, AND LIPIDS ON STAPHYLOCOCC...